Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4965643..4966140 Replicon chromosome
Accession NZ_CP066759
Organism Escherichia coli O157:H7 strain ECP19-198

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JG533_RS24690 Protein ID WP_001173343.1
Coordinates 4965643..4965816 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JG533_RS24695 Protein ID WP_000398619.1
Coordinates 4965844..4966140 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG533_RS24680 4962313..4963770 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
JG533_RS24685 4964007..4965524 + 1518 WP_001295074.1 lysine--tRNA ligase -
JG533_RS24690 4965643..4965816 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JG533_RS24695 4965844..4966140 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JG533_RS24700 4966367..4966639 - 273 WP_000405647.1 N-acetyltransferase -
JG533_RS24705 4966651..4966881 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
JG533_RS24710 4967062..4968693 + 1632 WP_001216477.1 sensor histidine kinase -
JG533_RS24715 4968690..4969409 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T184991 WP_001173343.1 NZ_CP066759:c4965816-4965643 [Escherichia coli O157:H7]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T184991 NZ_CP087859:1808802-1809080 [Moraxella bovis]
ATGATTCAAAGTTTTAAGTGCAAAGATGCCCAAAGCCTGTTTGAAACAGGGTCAAGCCGTGTATTTGCCAACTTTTTATC
CGTTGCCACTCGCAAACTTACCATGCTTGATAATGCCGTGGCACTTGCCGACCTACGCATTCCACCTGCCAACCGCCTTG
AAAAGCTGACAGGCGATAGAGAAGGTCAGTATAGTATTCGCATTAACGACCAATTTCGCATTTGCTTTGTGTGGGGCGAT
GATGGCGTGTATGATGTTGAGATTGTTGATTATCACTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT184991 WP_000398619.1 NZ_CP066759:c4966140-4965844 [Escherichia coli O157:H7]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT184991 NZ_CP087859:1809095-1809400 [Moraxella bovis]
ATGTTCAAAAATGGCATGAGACCTGTTCACGCAGGTGAGGTATTGCGTGAAGAATATCTAATCCCAATGGGTATGTCCGC
CACCGCCTTAGCAAATGCATTGGGCGTAACCCCTGCACGCATTAACGACATCGTGCGGCAGCGGCGTGGCGTTACGGCTG
ATACCGCTTTGCGTTTGGCTCGTTATTTTGGCGGTACGGCACAATTTTGGCTAAACTTACAAACCACCTATGAGCTTCGC
CTTGCCGAAACTACGCTTGGCGACAGTTTGGATTTTATCAGCCCTCATGAAGTTGCCCATGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References