Detailed information of TA system TA184987

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4619383..4619933
• Replicon: chromosome
• Accession: NZ_CP066759
• Organism: Escherichia coli O157:H7 strain ECP19-198

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: JG533_RS23140
• Protein ID: WP_001160975.1
• Coordinates: 4619383..4619697 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: JG533_RS23145
• Protein ID: WP_000125566.1
• Coordinates: 4619700..4619933 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG533_RS23115	4615058..4616344	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
JG533_RS23120	4616344..4617333	+	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
JG533_RS23125	4617330..4618151	+	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
JG533_RS23130	4618154..4618531	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
JG533_RS23135	4618538..4619386	+	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
JG533_RS23140	4619383..4619697	-	315	WP_001160975.1	CcdB family protein	Toxin
JG533_RS23145	4619700..4619933	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
JG533_RS23150	4620019..4620498	-	480	WP_000624372.1	type 3 dihydrofolate reductase	-
JG533_RS23155	4620690..4622552	-	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
JG533_RS23160	4622545..4623075	-	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
JG533_RS23165	4623183..4624514	-	1332	WP_001183177.1	MFS transporter	-
JG533_RS23170	4624572..4624859	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T184987 WP_001160975.1 NZ_CP066759:c4619697-4619383 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T184987 NZ_CP087859:1428728-1428910 [Moraxella bovis]
ATGAAGTATTCAGAGTTTCAAAGGTGGCTATTGGCACAAGGGGCAACGGTAAACAAGCAAGGCGGTAAAGGCTCGCACCG
TAAAGTTACCCTAAATGGCAAAACCACCACTTTCCCTTATCACGGCAGTAAAGAGATTGGGGAAGGACTAAGAAAGAAAA
TTTTAAAAGACTTGGATTTATAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT184987 WP_000125566.1 NZ_CP066759:c4619933-4619700 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT184987 NZ_CP087859:1428972-1429385 [Moraxella bovis]
ATGTACTATCCTGCTACTTTTACCCCTGATACCAATGATACTTTTTTGGTGGCGTTTCGTGATATTCCCGAAGCGGTGGG
CGTGGGTGAAACCTTTGAAATCGCCTACCAATCCGCCCTTGACGGCTTAGAAACGGCATTTAGCATCTATATGGACGAAC
GCAAGCCAATCCCCGCCCCCAGTGAGCTGTCTGACGGCGAACACGCCATTTATTTGCCTGTGGCGGTACAGACCAAACTT
GCCTTATATCACGAAATGCTAGCCCAAGGCGTTACTAAGGCGGAGCTGGCTCGCCGTCTGTCTGTCAATCAAAAACAAAT
TGATAGACTTTGGGACGTGAGCCACTCCACCAAATTAGAATTTTTGGAAAAGGCGTTTAGCGTGCTAGGCAAACGCCTAT
CCCTAGCCATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9