Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4089191..4089809 Replicon chromosome
Accession NZ_CP066759
Organism Escherichia coli O157:H7 strain ECP19-198

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JG533_RS20550 Protein ID WP_001291435.1
Coordinates 4089591..4089809 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JG533_RS20545 Protein ID WP_000344800.1
Coordinates 4089191..4089565 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG533_RS20535 4084280..4085473 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JG533_RS20540 4085496..4088645 + 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
JG533_RS20545 4089191..4089565 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JG533_RS20550 4089591..4089809 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
JG533_RS20555 4089981..4090532 + 552 WP_000102553.1 maltose O-acetyltransferase -
JG533_RS20560 4090648..4091118 + 471 WP_000136192.1 YlaC family protein -
JG533_RS20565 4091282..4092832 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JG533_RS20570 4092874..4093227 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JG533_RS20580 4093606..4093917 + 312 WP_000409911.1 MGMT family protein -
JG533_RS20585 4093950..4094522 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T184984 WP_001291435.1 NZ_CP066759:4089591-4089809 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T184984 NZ_CP087859:426668-426940 [Moraxella bovis]
GTGGCTAAACGTAGTTTTGAAATGGGCAATCCCTTTAAAAGGGATTTAAAAAAGCATTATTTGGCATTGATTGGCGAAGC
GTGGACGGAAGTGTCTGATTGCTTGTTAAATGCCAAACCCATACCTCAAAAATACCAAGACCACCCATTGCAAGGCGATT
GGGTGGGGTTTAGAGATTGTCATATCAAGCCTGATTTGGTGCTAATTTATAAAATAAATGATGAAGCGGTGGAATTACAT
CGGCTTGCCACGCATAGCGAGTTGTTTGGTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT184984 WP_000344800.1 NZ_CP066759:4089191-4089565 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT184984 NZ_CP087859:426382-426675 [Moraxella bovis]
ATGAGCCACACCAATTTTAATATGCGATTAGACAACGACCTACGCCAAAAAGCCTATCCTGTGCTAGAACAATACGGCTT
GACTCCAAGCCAAGCAGTGCGGATGTTTTTTAATCAAATCGCCCAAACAGGCAAAGTGCCATTGTCTTTTGATTGGGGGC
AGTCGCATACGCTTAGTAGCAATGGCGAAAAGCTGTTGCGTGAGAGTATTCAAGCTTTTGAAAATGGCGAAACCGAGCGA
TTTGATAGCTTAGATGAGTTAAATCAAGCAATGGCGGAGATTGCTCGTGGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References