Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2522728..2523366 Replicon chromosome
Accession NZ_CP066759
Organism Escherichia coli O157:H7 strain ECP19-198

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag JG533_RS12535 Protein ID WP_000813794.1
Coordinates 2522728..2522904 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag JG533_RS12540 Protein ID WP_001270286.1
Coordinates 2522950..2523366 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG533_RS12515 2518350..2519561 - 1212 WP_071525382.1 BenE family transporter YdcO -
JG533_RS12520 2519614..2520150 + 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
JG533_RS12525 2520223..2522184 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
JG533_RS12530 2522276..2522506 - 231 WP_000494244.1 YncJ family protein -
JG533_RS12535 2522728..2522904 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
JG533_RS12540 2522950..2523366 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
JG533_RS12545 2523445..2524851 + 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
JG533_RS12550 2525096..2526241 + 1146 WP_000047432.1 ABC transporter substrate-binding protein -
JG533_RS12555 2526259..2527272 + 1014 WP_000220402.1 ABC transporter ATP-binding protein -
JG533_RS12560 2527273..2528214 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T184980 WP_000813794.1 NZ_CP066759:2522728-2522904 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T184980 NZ_CP066759:2522728-2522904 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT184980 WP_001270286.1 NZ_CP066759:2522950-2523366 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT184980 NZ_CP066759:2522950-2523366 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCAGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References