Detailed information of TA system TA184969

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 890385..891039
• Replicon: chromosome
• Accession: NZ_CP066759
• Organism: Escherichia coli O157:H7 strain ECP19-198

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1PAM6
• Locus tag: JG533_RS04385
• Protein ID: WP_000244781.1
• Coordinates: 890632..891039 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: JG533_RS04380
• Protein ID: WP_000354048.1
• Coordinates: 890385..890651 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG533_RS04360	886473..887906	-	1434	WP_001310226.1	6-phospho-beta-glucosidase BglA	-
JG533_RS04365	887951..888262	+	312	WP_001182957.1	N(4)-acetylcytidine aminohydrolase	-
JG533_RS04370	888426..889085	+	660	WP_000250274.1	hemolysin III family protein	-
JG533_RS04375	889162..890142	-	981	WP_000886053.1	tRNA-modifying protein YgfZ	-
JG533_RS04380	890385..890651	+	267	WP_000354048.1	FAD assembly factor SdhE	Antitoxin
JG533_RS04385	890632..891039	+	408	WP_000244781.1	protein YgfX	Toxin
JG533_RS04390	891079..891600	-	522	WP_001055874.1	flavodoxin FldB	-
JG533_RS04395	891712..892608	+	897	WP_000806640.1	site-specific tyrosine recombinase XerD	-
JG533_RS04400	892633..893343	+	711	WP_000715214.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
JG533_RS04405	893349..895082	+	1734	WP_000813185.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16061.99 Da
• Isoelectric Point: 11.5202

>T184969 WP_000244781.1 NZ_CP066759:890632-891039 [Escherichia coli O157:H7]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T184969 NZ_CP087854:c2425701-2425429 [Moraxella bovis]
GTGGCTAAACGTAGTTTTGAAATGGGCAATCCCTTTAAAAGGGATTTAAAAAAGCATTATTTGGCATTGATTGGCGAAGC
GTGGACGGAAGTGTCTGATTGCTTGTTAAATGCCAAACCCATACCTCAAAAATACCAAGACCACCCATTGCAAGGCGATT
GGGTGGGGTTTAGAGATTGTCATATCAAGCCTGATTTGGTGCTAATTTATAAAATAAATGATGAAGCGGTGGAATTACAT
CGGCTTGCCACGCATAGCGAGTTGTTTGGTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10561.15 Da
• Isoelectric Point: 5.1700

>AT184969 WP_000354048.1 NZ_CP066759:890385-890651 [Escherichia coli O157:H7]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPELFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT184969 NZ_CP087854:c2425987-2425694 [Moraxella bovis]
ATGAGCCACACCAATTTTAATATGCGATTAGACAACGACCTACGCCAAAAAGCCTATCCTGTGCTAGAACAATACGGCTT
GACTCCAAGCCAAGCAGTGCGGATGTTTTTTAATCAAATCGCCCAAACAGGCAAAGTGCCATTGTCTTTTGATTGGGGGC
AGTCGCATACGCTTAGTAGCAATGGCGAAAAGCTGTTGCGTGAGAGTATTCAAGCTTTTGAAAATGGCGAAACCGAGCGA
TTTGATAGCTTAGATGAGTTAAATCAAGCAATGGCGGAGATTGCTCGTGGCTAA

Structures

Toxin

• AlphaFold DB: S1PAM6