Detailed information of TA system TA184965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PsyrTA/-
• Location: 375766..378094
• Replicon: chromosome
• Accession: NZ_CP066759
• Organism: Escherichia coli O157:H7 strain ECP19-198

Toxin (Protein)

• Gene name: PsyrT
• Uniprot ID: A0A6N0MHC8
• Locus tag: JG533_RS01790
• Protein ID: WP_000532146.1
• Coordinates: 375766..376740 (+)
• Length: 325 a.a.

Antitoxin (Protein)

• Gene name: PsyrA
• Uniprot ID: -
• Locus tag: JG533_RS01795
• Protein ID: WP_001303704.1
• Coordinates: 376799..378094 (+)
• Length: 432 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG533_RS01765	371623..372618	+	996	WP_000730263.1	gluconate operon transcriptional repressor GntR	-
JG533_RS01770	372757..373284	+	528	WP_000108330.1	gluconokinase	-
JG533_RS01775	373288..374628	+	1341	WP_000210111.1	gluconate transporter	-
JG533_RS01780	374768..375376	+	609	WP_000410808.1	ATP-dependent DNA helicase	-
JG533_RS01785	375361..375762	+	402	WP_000502498.1	ATP-dependent DNA helicase	-
JG533_RS01790	375766..376740	+	975	WP_000532146.1	ATP-dependent DNA helicase	Toxin
JG533_RS01795	376799..378094	+	1296	WP_001303704.1	DNA-protecting protein DprA	Antitoxin
JG533_RS01800	378140..378733	-	594	WP_001002544.1	NAAT family transporter YhgN	-
JG533_RS01805	378925..380028	+	1104	WP_000799956.1	aspartate-semialdehyde dehydrogenase	-
JG533_RS01810	380301..382487	+	2187	WP_001283734.1	1,4-alpha-glucan branching enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• DNA recombination-mediator protein A (115-429)

Sequences

Toxin

Protein

• Length: 325 a.a.
• Molecular weight: 36460.93 Da
• Isoelectric Point: 9.2667

>T184965 WP_000532146.1 NZ_CP066759:375766-376740 [Escherichia coli O157:H7]
VGRAGRGIDSAVGILLCGSEDRAIHKFFRESAFPAEAQIHEILNVLSVNDGLTLRGIEQRTNLRYGQIEKALKLLVAENP
SPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELADVESYITTKECKMQFLRRALDEPGAEHCGKCSSCLQHPLLSPDI
DSGLLHAANLFIKHADLSLNLNKQVAAGAFTQYGFKGNLPASLQGSTGRILSRWGYSGWGKQVAQEKKTGRFSDELVEAC
AEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFESTSTWFLILPGDWRRNLAYLLLMPLKKSWTIHRRKCSKTVSTSVKIS
TGRL

Nucleotide

• Length: 975 bp

>T184965 NZ_CP087854:1869835-1870113 [Moraxella bovis]
ATGATTCAAAGTTTTAAGTGCAAAGATGCCCAAAGCCTGTTTGAAACAGGGTCAAGCCGTGTATTTGCCAACTTTTTATC
CGTTGCCACTCGCAAACTTACCATGCTTGATAATGCCGTGGCACTTGCCGACCTACGCATTCCACCTGCCAACCGCCTTG
AAAAGCTGACAGGCGATAGAGAAGGTCAGTATAGTATTCGCATTAACGACCAATTTCGCATTTGCTTTGTGTGGGGCGAT
GATGGCGTGTATGATGTTGAGATTGTTGATTATCACTAA

Antitoxin

Protein

• Length: 432 a.a.
• Molecular weight: 47053.95 Da
• Isoelectric Point: 7.2590

>AT184965 WP_001303704.1 NZ_CP066759:376799-378094 [Escherichia coli O157:H7]
MDADSSNSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLLTSDFSRAAASKYKPLSNSEWGKFALWLKHQRISPAE
LLVPQPQEKLTGWSDPRISQERILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLI
AGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENG
ASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESISIERLASCT
GLTIEQISVWLNRAEEEGRVIRLGEGHYQFR

Nucleotide

• Length: 1296 bp

>AT184965 NZ_CP087854:1870143-1870433 [Moraxella bovis]
ATGAGACCTGTTCACGCAGGTGAGGTATTGCGTGAAGAATATCTAATCCCAATGGGTATGTCCGCCACCGCCTTAGCAAA
TGCATTGGGCGTAACCCCTGCACGCATTAACGACATCGTGCGGCAGCGGCGTGGCGTTACGGCTGATACCGCTTTGCGTT
TGGCTCGTTATTTTGGCGGTACGGCACAATTTTGGCTAAACTTACAAACCACCTATGAGCTTCGCCTTGCCGAAACTACG
CTTGGCGACAGTTTGGATTTTATCAGCCCTCATGAAGTTGCCCATGCCTAA

Structures

Toxin

• AlphaFold DB: A0A6N0MHC8