Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 351954..352754 Replicon chromosome
Accession NZ_CP066759
Organism Escherichia coli O157:H7 strain ECP19-198

Toxin (Protein)


Gene name ataT Uniprot ID A0A891SNC1
Locus tag JG533_RS01670 Protein ID WP_000342448.1
Coordinates 352227..352754 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag JG533_RS01665 Protein ID WP_001277108.1
Coordinates 351954..352220 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG533_RS01645 347612..348280 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
JG533_RS01650 348273..349331 + 1059 WP_001042018.1 permease-like cell division protein FtsX -
JG533_RS01655 349576..350430 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
JG533_RS01660 350701..351804 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
JG533_RS01665 351954..352220 + 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
JG533_RS01670 352227..352754 + 528 WP_000342448.1 GNAT family N-acetyltransferase Toxin
JG533_RS01675 352751..353134 - 384 WP_000778769.1 aspartate 1-decarboxylase autocleavage activator PanM -
JG533_RS01680 353558..354667 + 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
JG533_RS01685 354715..355641 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
JG533_RS01690 355638..356915 + 1278 WP_000803797.1 branched chain amino acid ABC transporter permease LivM -
JG533_RS01695 356912..357679 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19692.68 Da        Isoelectric Point: 7.3232

>T184964 WP_000342448.1 NZ_CP066759:352227-352754 [Escherichia coli O157:H7]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T184964 NZ_CP087854:1829592-1829870 [Moraxella bovis]
ATGATTTTTGAATGGGACGACAACAAAAATCGGACAAACAAGCAAAAGCACGGTGTCAGCTTTGAGAGTGCAAGCCGTAT
TTTTCTTGACCCATTTTTGATTGTTACAGAAGACACAAGTTCTGATGAAGTGCGGTGGCTTGGTGTGGGTAGTGTAGATG
GCGTGGTGATTTTACTCGTTGTACACACCCATACTGATACAGACGGTACAGAAATCATTCGCATTATCTCAGCACGCAAA
CTTAGCAGAGGAGAAATAGAACGCTATGGCTATTGTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT184964 WP_001277108.1 NZ_CP066759:351954-352220 [Escherichia coli O157:H7]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT184964 NZ_CP087854:1829857-1830144 [Moraxella bovis]
ATGGCTATTGTTAGAAAAACCTTAGACGATATCAAACACACACCCATTGATAAAGATGAATTGGCACAACTGCAATCAAT
GAGCGATGATAGCATTAACTACGATGATATTCCGCCATTAACTGACGTTCAAATTGCCAATTTGCGTTTGTACAAGCCCA
TTAAGCAACAAATCACATTAAGGCTTGACAGCGACATTGTGGCGTGGCTTAAAAAAGACGGCAAGGGTTATCAAACTCGT
GCTAATCAATTATTAAGACAGTTAATGTTGAAAGAATTAACCACATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6GTS

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References