Detailed information of TA system TA184962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 94863..95422
• Replicon: plasmid unnamed1
• Accession: NZ_CP066757
• Organism: Escherichia coli O157:H7 strain ECP19-798

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: JG530_RS27370
• Protein ID: WP_000421248.1
• Coordinates: 95147..95422 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: JG530_RS27365
• Protein ID: WP_001178089.1
• Coordinates: 94863..95147 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG530_RS27325	90054..90614	+	561	WP_000840472.1	fertility inhibition protein FinO	-
JG530_RS27330	90747..90959	+	213	WP_001302189.1	ANR family transcriptional regulator	-
JG530_RS27335	91204..91665	+	462	WP_001233853.1	thermonuclease family protein	-
JG530_RS27340	91711..91920	+	210	WP_001302200.1	hemolysin expression modulator Hha	-
JG530_RS27345	91958..92296	+	339	WP_000766796.1	hypothetical protein	-
JG530_RS27350	92536..92790	+	255	WP_000083831.1	replication regulatory protein RepA	-
JG530_RS27355	93026..93100	+	75	WP_001370046.1	RepA leader peptide Tap	-
JG530_RS27360	93093..93950	+	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
JG530_RS27365	94863..95147	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
JG530_RS27370	95147..95422	+	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JG530_RS27375	95517..95723	+	207	WP_001105064.1	hypothetical protein	-
JG530_RS27380	95823..96038	-	216	Protein_105	transposase	-
JG530_RS27385	96082..96648	-	567	Protein_106	IS3-like element IS600 family transposase	-
JG530_RS27390	96648..97064	-	417	Protein_107	IS91 family transposase	-
JG530_RS27395	97064..97249	-	186	WP_001302179.1	hypothetical protein	-
JG530_RS27400	97426..99636	+	2211	WP_000592771.1	catalase/peroxidase KatP	-
JG530_RS27405	99680..100069	+	390	WP_001172748.1	cytochrome b562 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyC / hlyA / hlyB / hlyD / toxB / toxB / toxB / toxB / katA / toxB / toxB / toxB / toxB / toxB / espP / stcE / exeE / exeG	1..127648	127648

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T184962 WP_000421248.1 NZ_CP066757:95147-95422 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T184962 NZ_CP087854:c1618303-1618046 [Moraxella bovis]
TTGAGGATTAGATGGTTTGATGAGGTATGGGAGGATTATTTGTATTGGCAAAGCCAAGATAAGAAAACCATAAAACGTAT
TAATACCTTGATTAAGGATTGCAGACGAGACCCTTTTGACGGTATCGGCAAACCCGAACCATTAAAATATAACCTAACAG
GCTACTGGTTAAGACGCATTAATGATGCCAACCGCTTGGTTTATTGTTGCGAAAGTGAAACACTCATCATTTCATGCCGT
CATCATTATGAGGCTTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT184962 WP_001178089.1 NZ_CP066757:94863-95147 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT184962 NZ_CP087854:c1618535-1618293 [Moraxella bovis]
ATGAATTATAGCGAATTTCGCCAAAACCTAGCCTCTGCCCTTGATTATGTTCAAGACAGCCATGCCCCTGTGATTGTCAA
ACGTGGCAAACATTCTGCGGTGGTGATTAGTCTTGATGAATATAATGCTTTTAAAGAAACAGATTATCTATTGTCAAACC
CTGCTAATGCCGAACACCTTATGCGTGGCGTGCAAGCGGTAAAAAATCGTCAACTTACCCAAAGGGATTTGATTGAGGAT
TAG

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8