Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4956064..4956561 Replicon chromosome
Accession NZ_CP066756
Organism Escherichia coli O157:H7 strain ECP19-798

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JG530_RS24595 Protein ID WP_001173343.1
Coordinates 4956064..4956237 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JG530_RS24600 Protein ID WP_000398619.1
Coordinates 4956265..4956561 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG530_RS24585 4952734..4954191 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
JG530_RS24590 4954428..4955945 + 1518 WP_001295074.1 lysine--tRNA ligase -
JG530_RS24595 4956064..4956237 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JG530_RS24600 4956265..4956561 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JG530_RS24605 4956788..4957060 - 273 WP_000405647.1 N-acetyltransferase -
JG530_RS24610 4957072..4957302 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
JG530_RS24615 4957483..4959114 + 1632 WP_001216477.1 sensor histidine kinase -
JG530_RS24620 4959111..4959830 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T184959 WP_001173343.1 NZ_CP066756:c4956237-4956064 [Escherichia coli O157:H7]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T184959 NZ_CP087851:2636274-2636609 [Moraxella bovis]
ATGTTGCATTTTGCTGAAACATCATTATTCACTAAACAAATCATAGACTTAATGGATGATGACGAGTACAGACTATTACA
AACAAACTTGATGAAAAACCCACATCAAGGCGACATTGTTCGTGGTACTGGCGGAGTACGCAAAACAAGATGGTCAATTG
ATGGCAAGGGCAAAAGTGGTGGCAGTCGCATCATTTACTTTTTTGTTGATGGTGCTGGCATTTTCTTTATGCTCTTGGCT
TATCCTAAATCAAAACAAACAACGCTATCGGCGGATGAGAAAAAAGAAATGCTCAAACTAACCACCGCAATTAAGGAGAT
TTACCGTGCAAAATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT184959 WP_000398619.1 NZ_CP066756:c4956561-4956265 [Escherichia coli O157:H7]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT184959 NZ_CP087851:2636599-2636880 [Moraxella bovis]
GTGCAAAATGAGTTTTACGATGATTTAAAAGCCTCATTGACTGAGGCTCTGGCAATCGCTAAGGGCGAAATTTCCCCAAG
TCGTACATTCACTTATGAACGCCCAAACATCAAAGATATTCGGGCAAAAACAGGGCTATCACAAACCCAGTTCGCCCAGA
AGCTACACATAAGCCCTAAAACGCTTAAAAACTGGGAACAGGGCATTAGAACGCCAACAGGTCCAGCCATTACCCTAATT
AGATTACTTGACAAAAACCCTGATTTAATTAGCATGGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References