Detailed information of TA system TA184958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4859073..4859668
• Replicon: chromosome
• Accession: NZ_CP066756
• Organism: Escherichia coli O157:H7 strain ECP19-798

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: JG530_RS24095
• Protein ID: WP_000239579.1
• Coordinates: 4859073..4859423 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: JG530_RS24100
• Protein ID: WP_001223210.1
• Coordinates: 4859417..4859668 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG530_RS24075	4854519..4855541	-	1023	WP_001301928.1	ABC transporter permease	-
JG530_RS24080	4855555..4857057	-	1503	WP_000205806.1	sugar ABC transporter ATP-binding protein	-
JG530_RS24085	4857197..4858153	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
JG530_RS24090	4858463..4858993	+	531	WP_000055075.1	inorganic diphosphatase	-
JG530_RS24095	4859073..4859423	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
JG530_RS24100	4859417..4859668	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
JG530_RS24105	4859880..4860221	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
JG530_RS24110	4860224..4864003	-	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T184958 WP_000239579.1 NZ_CP066756:c4859423-4859073 [Escherichia coli O157:H7]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T184958 NZ_CP087851:c2301743-2301471 [Moraxella bovis]
GTGGCTAAACGTAGTTTTGAAATGGGCAATCCCTTTAAAAGGGATTTAAAAAAGCATTATTTGGCATTGATTGGCGAAGC
GTGGACGGAAGTGTCTGATTGCTTGTTAAATGCCAAACCCATACCTCAAAAATACCAAGACCACCCATTGCAAGGCGATT
GGGTGGGGTTTAGAGATTGTCATATCAAGCCTGATTTGGTGCTAATTTATAAAATAAATGATGAAGCGGTGGAATTACAT
CGGCTTGCCACGCATAGCGAGTTGTTTGGTTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT184958 WP_001223210.1 NZ_CP066756:c4859668-4859417 [Escherichia coli O157:H7]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT184958 NZ_CP087851:c2302029-2301736 [Moraxella bovis]
ATGAGCCACACCAATTTTAATATGCGATTAGACAACGACCTACGCCAAAAAGCCTATCCTGTGCTAGAACAATACGGCTT
GACTCCAAGCCAAGCAGTGCGGATGTTTTTTAATCAAATCGCCCAAACAGGCAAAGTGCCATTGTCTTTTGATTGGGGGC
AGTCGCATACGCTTAGTAGCAATGGCGAAAAGCTGTTGCGTGAGAGTATTCAAGCTTTTGAAAATGGCGAAACCGAGCGA
TTTGATAGCTTAGATGAGTTAAATCAAGCAATGGCGGAGATTGCTCGTGGCTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8