Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4118542..4119160 Replicon chromosome
Accession NZ_CP066756
Organism Escherichia coli O157:H7 strain ECP19-798

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JG530_RS20755 Protein ID WP_001291435.1
Coordinates 4118942..4119160 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JG530_RS20750 Protein ID WP_000344800.1
Coordinates 4118542..4118916 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG530_RS20740 4113631..4114824 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JG530_RS20745 4114847..4117996 + 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
JG530_RS20750 4118542..4118916 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JG530_RS20755 4118942..4119160 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
JG530_RS20760 4119332..4119883 + 552 WP_000102553.1 maltose O-acetyltransferase -
JG530_RS20765 4119999..4120469 + 471 WP_000136192.1 YlaC family protein -
JG530_RS20770 4120633..4122183 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JG530_RS20775 4122225..4122578 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JG530_RS20785 4122957..4123268 + 312 WP_000409911.1 MGMT family protein -
JG530_RS20790 4123301..4123873 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T184952 WP_001291435.1 NZ_CP066756:4118942-4119160 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T184952 NZ_CP087851:c965792-965514 [Moraxella bovis]
ATGATTTTTGAATGGGACGACAACAAAAATCGGACAAACAAGCAAAAGCACGGTGTCAGCTTTGAGAGTGCAAGCCGTAT
TTTTCTTGACCCATTTTTGATTGTTACAGAAGACACAAGTTCTGATGAAGTGCGGTGGCTTGGTGTGGGTAGTGTAGATG
GCGTGGTGATTTTACTCGTTGTACACACCCATACTGATACAGACGGTACAGAAATCATTCGCATTATCTCAGCACGCAAA
CTTAGCAGAGGAGAAATAGAACGCTATGGCTATTGTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT184952 WP_000344800.1 NZ_CP066756:4118542-4118916 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT184952 NZ_CP087851:c965527-965240 [Moraxella bovis]
ATGGCTATTGTTAGAAAAACCTTAGACGATATCAAACACACACCCATTGATAAAGATGAATTGGCACAACTGCAATCAAT
GAGCGATGATAGCATTAACTACGATGATATTCCGCCATTAACTGACGTTCAAATTGCCAATTTGCGTTTGTACAAGCCCA
TTAAGCAACAAATCACATTAAGGCTTGACAGCGACATTGTGGCGTGGCTTAAAAAAGACGGCAAGGGTTATCAAACTCGT
GCTAATCAATTATTAAGACAGTTAATGTTGAAAGAATTAACCACATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References