Detailed information of TA system TA184949

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3205463..3206258
• Replicon: chromosome
• Accession: NZ_CP066756
• Organism: Escherichia coli O157:H7 strain ECP19-798

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: JG530_RS16305
• Protein ID: WP_000854914.1
• Coordinates: 3205463..3205837 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0B0VS41
• Locus tag: JG530_RS16310
• Protein ID: WP_001280954.1
• Coordinates: 3205884..3206258 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG530_RS16265	3200467..3200958	-	492	WP_001301587.1	DUF1097 domain-containing protein	-
JG530_RS16270	3201060..3201614	-	555	WP_001001917.1	molecular chaperone YcdY	-
JG530_RS16275	3201638..3202375	-	738	WP_000283664.1	zinc-binding phosphatase	-
JG530_RS16280	3202430..3203368	-	939	WP_000351284.1	glyoxylate/hydroxypyruvate reductase GhrA	-
JG530_RS16290	3203839..3204681	-	843	WP_001280481.1	DUF4942 domain-containing protein	-
JG530_RS16295	3204766..3204963	-	198	WP_000839282.1	DUF957 domain-containing protein	-
JG530_RS16300	3204975..3205466	-	492	WP_000976853.1	hypothetical protein	-
JG530_RS16305	3205463..3205837	-	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
JG530_RS16310	3205884..3206258	-	375	WP_001280954.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JG530_RS16315	3206421..3206642	-	222	WP_001220314.1	DUF987 domain-containing protein	-
JG530_RS16320	3206705..3207181	-	477	WP_001186738.1	RadC family protein	-
JG530_RS16325	3207197..3207682	-	486	WP_000214398.1	antirestriction protein	-
JG530_RS16330	3207773..3208591	-	819	WP_001234682.1	DUF945 domain-containing protein	-
JG530_RS16335	3208931..3210001	-	1071	WP_000102669.1	patatin-like phospholipase family protein	-
JG530_RS16340	3209998..3210903	-	906	WP_000203541.1	chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T184949 WP_000854914.1 NZ_CP066756:c3205837-3205463 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T184949 NZ_CP087851:638625-639026 [Moraxella bovis]
ATGTTTAGTTTTATGCTGGATACCAATATCTACATTTATGTGATAAAAGAACGCCCCATAAAAGCATTAAACAAATTTAA
CCAATATGCAGGGCGGATTTGCGTAAGCAGTATCGTGGCAAGCGAGTTGTATTTTGGGGCGTACAACAGCCAATTTATGG
ATAAAAATCTACATCAAGTCGAAGATTTTTTATCACGTTTGACGGTGCTTGATTATACCGCAGAATGCTCGCCCCATTAC
GGCGAGATTTGTGCTGATTTGACCAAGCAGGGCAAGCTCATTAGTGAAAATGACATTCATATCGCCTCTCATGCACGGGC
ATTGGGGCTGACCTTGATTACCAATAATACCGATGAATTTAAACGAGTGTCAGGGCTTAGGATTGGTAATTGGGCGATTT
GA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13718.51 Da
• Isoelectric Point: 6.6249

>AT184949 WP_001280954.1 NZ_CP066756:c3206258-3205884 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT184949 NZ_CP087851:638377-638610 [Moraxella bovis]
ATGCAAACCAGTGTTTTTTTTAATAATCGCACCCAAGCGGTTCGCATTCCTGCCAAATTTCGCTTTGATGACGATGTCAA
AGCCGTCATGATTAGAAAAGTGGGCAATGAGCGGATTTTGACCCCAATTGATGGTGTGTGGGACAGCTTTTTTTTGAGTG
ATGAGCGAGCGAGTGATGATTTTATGGCAAACCGCCCGACCATGCCAAAAAGCGAGCGATTGACTTTTGACTGA

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5

Antitoxin

• AlphaFold DB: A0A0B0VS41