Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2633495..2634133 Replicon chromosome
Accession NZ_CP066756
Organism Escherichia coli O157:H7 strain ECP19-798

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag JG530_RS13130 Protein ID WP_000813794.1
Coordinates 2633957..2634133 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag JG530_RS13125 Protein ID WP_001270286.1
Coordinates 2633495..2633911 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG530_RS13105 2628647..2629588 - 942 WP_001251315.1 ABC transporter permease -
JG530_RS13110 2629589..2630602 - 1014 WP_000220402.1 ABC transporter ATP-binding protein -
JG530_RS13115 2630620..2631765 - 1146 WP_000047432.1 ABC transporter substrate-binding protein -
JG530_RS13120 2632010..2633416 - 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
JG530_RS13125 2633495..2633911 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
JG530_RS13130 2633957..2634133 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
JG530_RS13135 2634355..2634585 + 231 WP_000494244.1 YncJ family protein -
JG530_RS13140 2634677..2636638 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
JG530_RS13145 2636711..2637247 - 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
JG530_RS13150 2637300..2638511 + 1212 WP_071525382.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T184948 WP_000813794.1 NZ_CP066756:c2634133-2633957 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T184948 NZ_CP087851:477801-477989 [Moraxella bovis]
ATGCCCCCAAGTAGCGATGAGATGATAAAGCGAATTACCGAAGACGGTTGGTATTTTGTAAAACAAAAAGGCTCTCACAA
GCACTACAAACACCCAACCAAAGAAGGTAAAGTAACAATCCCAAACGGTCGTGAAAACCTACCCAAAGGAACGCTAAAAA
GCATCATCAAACAAGCAGGGTTGGATTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT184948 WP_001270286.1 NZ_CP066756:c2633911-2633495 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT184948 NZ_CP087851:478052-478459 [Moraxella bovis]
ATGTTATTTCCCATTGCCATTGAAAAACCTGCCAATGAAAACGAATGTTATGGCGTGATTGTGCCAGACATTGCAGGGTG
TTTTAGTGCAGGCGATACACCTGATGAAGCCATTAAGAATGCTTATGAAGCCATCAGTTTGCATTTGGAAGGCATGGCAG
AAGATGGCGATACACTACCTACCGCCAAGCCCATTTTTTATCATCAAGACAATAGCGAATTTGACGGTATGTTGTGGGCA
GTGGTGGACGTGGATATGTCTGCCTTTATGGGTAAGGCGGAGAAAATCAACGTTACCTTGCCAAGCCTACTCATCACCAA
AATTGACGAAAAAGTCTTGTCTCATAAATCGCTATACAAAAGCCGTTCTAATTATTTGGCACAATTGGCGATGGCAGATT
TGGGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References