Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1034332..1034915 Replicon chromosome
Accession NZ_CP066756
Organism Escherichia coli O157:H7 strain ECP19-798

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag JG530_RS05040 Protein ID WP_000254738.1
Coordinates 1034580..1034915 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag JG530_RS05035 Protein ID WP_000581937.1
Coordinates 1034332..1034580 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG530_RS05025 1030671..1031972 + 1302 WP_000046824.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
JG530_RS05030 1032020..1034254 + 2235 WP_000226815.1 GTP pyrophosphokinase -
JG530_RS05035 1034332..1034580 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
JG530_RS05040 1034580..1034915 + 336 WP_000254738.1 endoribonuclease MazF Toxin
JG530_RS05045 1034986..1035777 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
JG530_RS05050 1036005..1037642 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
JG530_RS05055 1037730..1039028 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T184938 WP_000254738.1 NZ_CP066756:1034580-1034915 [Escherichia coli O157:H7]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T184938 NZ_CP087848:433805-434077 [Moraxella bovis]
GTGGCTAAACGTAGTTTTGAAATGGGCAATCCCTTTAAAAGGGATTTAAAAAAGCATTATTTGGCATTGATTGGCGAAGC
GTGGACGGAAGTGTCTGATTGCTTGTTAAATGCCAAACCCATACCTCAAAAATACCAAGACCACCCATTGCAAGGCGATT
GGGTGGGGTTTAGAGATTGTCATATCAAGCCTGATTTGGTGCTAATTTATAAAATAAATGATGAAGCGGTGGAATTACAT
CGGCTTGCCACGCATAGCGAGTTGTTTGGTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT184938 WP_000581937.1 NZ_CP066756:1034332-1034580 [Escherichia coli O157:H7]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT184938 NZ_CP087848:433519-433812 [Moraxella bovis]
ATGAGCCACACCAATTTTAATATGCGATTAGACAACGACCTACGCCAAAAAGCCTATCCTGTGCTAGAACAATACGGCTT
GACTCCAAGCCAAGCAGTGCGGATGTTTTTTAATCAAATCGCCCAAACAGGCAAAGTGCCATTGTCTTTTGATTGGGGGC
AGTCGCATACGCTTAGTAGCAATGGCGAAAAGCTGTTGCGTGAGAGTATTCAAGCTTTTGAAAATGGCGAAACCGAGCGA
TTTGATAGCTTAGATGAGTTAAATCAAGCAATGGCGGAGATTGCTCGTGGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References