Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 888851..889505 Replicon chromosome
Accession NZ_CP066756
Organism Escherichia coli O157:H7 strain ECP19-798

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag JG530_RS04385 Protein ID WP_000244781.1
Coordinates 889098..889505 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JG530_RS04380 Protein ID WP_000354046.1
Coordinates 888851..889117 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG530_RS04360 884939..886372 - 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -
JG530_RS04365 886417..886728 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
JG530_RS04370 886892..887551 + 660 WP_000250274.1 hemolysin III family protein -
JG530_RS04375 887628..888608 - 981 WP_000886053.1 tRNA-modifying protein YgfZ -
JG530_RS04380 888851..889117 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JG530_RS04385 889098..889505 + 408 WP_000244781.1 protein YgfX Toxin
JG530_RS04390 889545..890066 - 522 WP_001055874.1 flavodoxin FldB -
JG530_RS04395 890178..891074 + 897 WP_000806640.1 site-specific tyrosine recombinase XerD -
JG530_RS04400 891099..891809 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JG530_RS04405 891815..893548 + 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T184937 WP_000244781.1 NZ_CP066756:889098-889505 [Escherichia coli O157:H7]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T184937 NZ_CP087848:c100649-100314 [Moraxella bovis]
ATGTTGCATTTTGCTGAAACATCATTATTCACTAAACAAATCATAGACTTAATGGATGATGACGAGTACAGACTATTACA
AACAAACTTGATGAAAAACCCACATCAAGGCGACATTGTTCGTGGTACTGGCGGAGTACGCAAAACAAGATGGTCAATTG
ATGGCAAGGGCAAAAGTGGTGGCAGTCGCATCATTTACTTTTTTGTTGATGGTGCTGGCATTTTCTTTATGCTCTTGGCT
TATCCTAAATCAAAACAAACAACGCTATCGGCGGATGAGAAAAAAGAAATGCTCAAACTAACCACCGCAATTAAGGAGAT
TTACCGTGCAAAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT184937 WP_000354046.1 NZ_CP066756:888851-889117 [Escherichia coli O157:H7]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT184937 NZ_CP087848:c100324-100043 [Moraxella bovis]
GTGCAAAATGAGTTTTACGATGATTTAAAAGCCTCATTGACTGAGGCTCTGGCAATCGCTAAGGGCGAAATTTCCCCAAG
TCGTACATTCACTTATGAACGCCCAAACATCAAAGATATTCGGGCAAAAACAGGGCTATCACAAACCCAGTTCGCCCAGA
AGCTACACATAAGCCCTAAAACGCTTAAAAACTGGGAACAGGGCATTAGAACGCCAACAGGTCCAGCCATTACCCTAATT
AGATTACTTGACAAAAACCCTGATTTAATTAGCATGGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References