Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 309306..309937 Replicon chromosome
Accession NZ_CP066756
Organism Escherichia coli O157:H7 strain ECP19-798

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag JG530_RS01420 Protein ID WP_001259386.1
Coordinates 309306..309581 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag JG530_RS01425 Protein ID WP_000593555.1
Coordinates 309578..309937 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG530_RS01405 304310..305377 + 1068 WP_000361458.1 HlyD family secretion protein -
JG530_RS01410 305374..308109 + 2736 WP_032247297.1 ribosome-associated ATPase/putative transporter RbbA -
JG530_RS01415 308109..309233 + 1125 WP_001301659.1 ABC transporter permease -
JG530_RS01420 309306..309581 + 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
JG530_RS01425 309578..309937 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JG530_RS01430 309987..310847 - 861 WP_001131788.1 class II aldolase -
JG530_RS01435 310879..311148 - 270 WP_000933060.1 HPr family phosphocarrier protein -
JG530_RS01440 311138..312646 - 1509 WP_001301773.1 carbohydrate kinase -
JG530_RS01445 312639..313997 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
JG530_RS01450 314074..314355 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
JG530_RS01455 314352..314825 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T184931 WP_001259386.1 NZ_CP066756:309306-309581 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T184931 NZ_CP087844:1155102-1155359 [Moraxella bovis]
TTGAGGATTAGATGGTTTGATGAGGTATGGGAGGATTATTTGTATTGGCAAAGCCAAGATAAGAAAACCATAAAACGTAT
TAATACCTTGATTAAGGATTGCAGACGAGACCCTTTTGACGGTATCGGCAAACCCGAACCATTAAAATATAACCTAACAG
GCTACTGGTTAAGACGCATTGATGATGCCAACCGCTTGGTTTATTGTTGCGAAAGTGAAACACTCATCATTTCATGCCGT
CATCATTATGAGGCTTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT184931 WP_000593555.1 NZ_CP066756:309578-309937 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT184931 NZ_CP087844:1154870-1155112 [Moraxella bovis]
ATGAATTATAGCGAATTTCGCCAAAACCTAGCGTCCGCCCTTGATTATGTTCAAGACAGCCATGCCCCTGTGATTGTCAA
ACGTGGCAAACATTCTGCGGTGGTGATTAGTCTTGATGAATATAATGCTTTTAAAGAAACAGATTATCTATTGTCAAACC
CTGCTAATGCCGAACACCTTATGCGTGGCGTGCAAGCGGTAAAAAATCGTCAACTTACCCAAAGGGATTTGATTGAGGAT
TAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References