Detailed information of TA system TA184930

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 77305..77864
• Replicon: plasmid unnamed2
• Accession: NZ_CP066755
• Organism: Escherichia coli O157:H7 strain ECP19-598

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: JG531_RS28490
• Protein ID: WP_000421248.1
• Coordinates: 77589..77864 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: JG531_RS28485
• Protein ID: WP_001178089.1
• Coordinates: 77305..77589 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG531_RS28445	72496..73056	+	561	WP_000840472.1	fertility inhibition protein FinO	-
JG531_RS28450	73189..73401	+	213	WP_001302189.1	ANR family transcriptional regulator	-
JG531_RS28455	73646..74107	+	462	WP_001233853.1	thermonuclease family protein	-
JG531_RS28460	74153..74362	+	210	WP_001302200.1	hemolysin expression modulator Hha	-
JG531_RS28465	74400..74738	+	339	WP_000766796.1	hypothetical protein	-
JG531_RS28470	74978..75232	+	255	WP_000083831.1	replication regulatory protein RepA	-
JG531_RS28475	75468..75542	+	75	WP_001370046.1	RepA leader peptide Tap	-
JG531_RS28480	75535..76392	+	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
JG531_RS28485	77305..77589	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
JG531_RS28490	77589..77864	+	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JG531_RS28495	77959..78165	+	207	WP_001105064.1	hypothetical protein	-
JG531_RS28500	78265..78480	-	216	Protein_86	transposase	-
JG531_RS28505	78524..79090	-	567	Protein_87	IS3-like element IS600 family transposase	-
JG531_RS28510	79090..79506	-	417	Protein_88	IS91 family transposase	-
JG531_RS28515	79506..79691	-	186	WP_001302179.1	hypothetical protein	-
JG531_RS28520	79868..82078	+	2211	WP_000592771.1	catalase/peroxidase KatP	-
JG531_RS28525	82122..82511	+	390	WP_001172748.1	cytochrome b562 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyA / hlyB / hlyD / toxB / toxB / toxB / toxB / toxB / toxB / toxB / espP / stcE / exeE / exeG / hlyC	1..114710	114710

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T184930 WP_000421248.1 NZ_CP066755:77589-77864 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T184930 NZ_CP087844:1154870-1155112 [Moraxella bovis]
ATGAATTATAGCGAATTTCGCCAAAACCTAGCGTCCGCCCTTGATTATGTTCAAGACAGCCATGCCCCTGTGATTGTCAA
ACGTGGCAAACATTCTGCGGTGGTGATTAGTCTTGATGAATATAATGCTTTTAAAGAAACAGATTATCTATTGTCAAACC
CTGCTAATGCCGAACACCTTATGCGTGGCGTGCAAGCGGTAAAAAATCGTCAACTTACCCAAAGGGATTTGATTGAGGAT
TAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT184930 WP_001178089.1 NZ_CP066755:77305-77589 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT184930 NZ_CP087844:1155102-1155359 [Moraxella bovis]
TTGAGGATTAGATGGTTTGATGAGGTATGGGAGGATTATTTGTATTGGCAAAGCCAAGATAAGAAAACCATAAAACGTAT
TAATACCTTGATTAAGGATTGCAGACGAGACCCTTTTGACGGTATCGGCAAACCCGAACCATTAAAATATAACCTAACAG
GCTACTGGTTAAGACGCATTGATGATGCCAACCGCTTGGTTTATTGTTGCGAAAGTGAAACACTCATCATTTCATGCCGT
CATCATTATGAGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8