Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 51766..52325 | Replicon | plasmid unnamed2 |
| Accession | NZ_CP066755 | ||
| Organism | Escherichia coli O157:H7 strain ECP19-598 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | O82928 |
| Locus tag | JG531_RS28345 | Protein ID | WP_000421248.1 |
| Coordinates | 52050..52325 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | C1J8K5 |
| Locus tag | JG531_RS28340 | Protein ID | WP_001178089.1 |
| Coordinates | 51766..52050 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JG531_RS28300 | 46957..47517 | + | 561 | WP_000840472.1 | fertility inhibition protein FinO | - |
| JG531_RS28305 | 47650..47862 | + | 213 | WP_001302189.1 | ANR family transcriptional regulator | - |
| JG531_RS28310 | 48107..48568 | + | 462 | WP_001233853.1 | thermonuclease family protein | - |
| JG531_RS28315 | 48614..48823 | + | 210 | WP_001302200.1 | hemolysin expression modulator Hha | - |
| JG531_RS28320 | 48861..49199 | + | 339 | WP_000766796.1 | hypothetical protein | - |
| JG531_RS28325 | 49439..49693 | + | 255 | WP_000083831.1 | replication regulatory protein RepA | - |
| JG531_RS28330 | 49929..50003 | + | 75 | WP_001370046.1 | RepA leader peptide Tap | - |
| JG531_RS28335 | 49996..50853 | + | 858 | WP_000130945.1 | incFII family plasmid replication initiator RepA | - |
| JG531_RS28340 | 51766..52050 | + | 285 | WP_001178089.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| JG531_RS28345 | 52050..52325 | + | 276 | WP_000421248.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| JG531_RS28350 | 52420..52626 | + | 207 | WP_001105064.1 | hypothetical protein | - |
| JG531_RS28355 | 52726..52941 | - | 216 | Protein_57 | transposase | - |
| JG531_RS28360 | 52985..53551 | - | 567 | Protein_58 | IS3-like element IS600 family transposase | - |
| JG531_RS28365 | 53551..53967 | - | 417 | Protein_59 | IS91 family transposase | - |
| JG531_RS28370 | 53967..54152 | - | 186 | WP_001302179.1 | hypothetical protein | - |
| JG531_RS28375 | 54329..56539 | + | 2211 | WP_000592771.1 | catalase/peroxidase KatP | - |
| JG531_RS28380 | 56583..56972 | + | 390 | WP_001172748.1 | cytochrome b562 family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | hlyA / hlyB / hlyD / toxB / toxB / toxB / toxB / toxB / toxB / toxB / espP / stcE / exeE / exeG / hlyC | 1..114710 | 114710 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10735.42 Da Isoelectric Point: 9.9324
>T184929 WP_000421248.1 NZ_CP066755:52050-52325 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T184929 NZ_CP087844:c961022-960744 [Moraxella bovis]
ATGATTTTTGAATGGGACGACAACAAAAATCGGACAAACAAGCAAAAGCACGGTGTCAGCTTTGAGAGTGCAAGCCGTAT
TTTTCTTGACCCATTTTTGATTGTTACAGAAGACACAAGTTCTGATGAAGTGCGGTGGCTTGGTGTGGGTAGTGTAGATG
GCGTGGTGATTTTACTCGTTGTACACACCCATACTGATACAGACGGTACAGAAATCATTCGCATTATCTCAGCACGCAAA
CTTAGCAGAGGAGAAATAGAACGCTATGGCTATTGTTAG
ATGATTTTTGAATGGGACGACAACAAAAATCGGACAAACAAGCAAAAGCACGGTGTCAGCTTTGAGAGTGCAAGCCGTAT
TTTTCTTGACCCATTTTTGATTGTTACAGAAGACACAAGTTCTGATGAAGTGCGGTGGCTTGGTGTGGGTAGTGTAGATG
GCGTGGTGATTTTACTCGTTGTACACACCCATACTGATACAGACGGTACAGAAATCATTCGCATTATCTCAGCACGCAAA
CTTAGCAGAGGAGAAATAGAACGCTATGGCTATTGTTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10474.89 Da Isoelectric Point: 5.6558
>AT184929 WP_001178089.1 NZ_CP066755:51766-52050 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
Download Length: 285 bp
>AT184929 NZ_CP087844:c960757-960470 [Moraxella bovis]
ATGGCTATTGTTAGAAAAACCTTAGACGATATCAAACACACACCCATTGATAAAGATGAATTGGCACAACTGCAATCAAT
GAGCGATGATAGCATTAACTACGATGATATTCCGCCATTAACTGACGTTCAAATTGCCAATTTGCGTTTGTACAAGCCCA
TTAAGCAACAAATCACATTAAGGCTTGACAGCGACATTGTGGCGTGGCTTAAAAAAGACGGCAAGGGTTATCAAACTCGT
GCTAATCAATTATTAAGACAGTTAATGTTGAAAGAATTAACCACATAA
ATGGCTATTGTTAGAAAAACCTTAGACGATATCAAACACACACCCATTGATAAAGATGAATTGGCACAACTGCAATCAAT
GAGCGATGATAGCATTAACTACGATGATATTCCGCCATTAACTGACGTTCAAATTGCCAATTTGCGTTTGTACAAGCCCA
TTAAGCAACAAATCACATTAAGGCTTGACAGCGACATTGTGGCGTGGCTTAAAAAAGACGGCAAGGGTTATCAAACTCGT
GCTAATCAATTATTAAGACAGTTAATGTTGAAAGAATTAACCACATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z6FAZ0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A731IHA8 |