Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 12071..12596 Replicon plasmid unnamed2
Accession NZ_CP066755
Organism Escherichia coli O157:H7 strain ECP19-598

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag JG531_RS28125 Protein ID WP_001159868.1
Coordinates 12291..12596 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag JG531_RS28120 Protein ID WP_000813634.1
Coordinates 12071..12289 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG531_RS28090 7354..7584 + 231 WP_010891288.1 hypothetical protein -
JG531_RS28095 7705..8445 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
JG531_RS28100 8730..9707 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
JG531_RS28105 10115..10315 - 201 WP_000708307.1 hypothetical protein -
JG531_RS28110 10312..10932 - 621 WP_001248529.1 hypothetical protein -
JG531_RS28115 10929..11612 - 684 WP_010891291.1 DNA-binding protein -
JG531_RS28120 12071..12289 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JG531_RS28125 12291..12596 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
JG531_RS28130 12597..13403 + 807 WP_000016990.1 site-specific integrase -
JG531_RS28140 16079..17245 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyA / hlyB / hlyD / toxB / toxB / toxB / toxB / toxB / toxB / toxB / espP / stcE / exeE / exeG / hlyC 1..114710 114710


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T184928 WP_001159868.1 NZ_CP066755:12291-12596 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T184928 NZ_CP087844:c921571-921293 [Moraxella bovis]
ATGATTCAAAGTTTTAAGTGCAAAGATGCCCAAAGCCTGTTTGAAACAGGGTCAAGCCGTGTATTTGCCAACTTTTTATC
CGTTGCCACTCGCAAACTTACCATGCTTGATAATGCCGTGGCACTTGCCGACCTACGCATTCCACCTGCCAACCGCCTTG
AAAAGCTGACAGGCGATAGAGAAGGTCAGTATAGTATTCGCATTAACGACCAATTTCGCATTTGCTTTGTGTGGGGCGAT
GATGGCGTGTATGATGTTGAGATTGTTGATTATCACTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT184928 WP_000813634.1 NZ_CP066755:12071-12289 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT184928 NZ_CP087844:c921278-920973 [Moraxella bovis]
ATGTTCAAAAATGGCATGAGACCTGTTCACGCAGGTGAGGTATTGCGTGAAGAATATCTAATCCCAATGGGTATGTCCGC
CACCGCCTTAGCAAATGCATTGGGCGTAACCCCTGCACGCATTAACGACATCGTGCGGCAGCGGCGTGGCGTTACGGCTG
ATACCGCTTTGCGTTTGGCTCGTTATTTTGGCGGTACGGCACAATTTTGGCTAAACTTACAAACCACCTATGAGCTTCGC
CTTGCCGAAACTACGCTTGGCGACAGTTTGGATTTTATCAGCCCTCATGAAGTTGCCCATGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References