Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5056936..5057433 Replicon chromosome
Accession NZ_CP066753
Organism Escherichia coli O157:H7 strain ECP19-598

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JG531_RS25195 Protein ID WP_001173343.1
Coordinates 5056936..5057109 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JG531_RS25200 Protein ID WP_000398619.1
Coordinates 5057137..5057433 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG531_RS25185 5053606..5055063 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
JG531_RS25190 5055300..5056817 + 1518 WP_001295074.1 lysine--tRNA ligase -
JG531_RS25195 5056936..5057109 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JG531_RS25200 5057137..5057433 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JG531_RS25205 5057660..5057932 - 273 WP_000405647.1 N-acetyltransferase -
JG531_RS25210 5057944..5058174 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
JG531_RS25215 5058355..5059986 + 1632 WP_001216477.1 sensor histidine kinase -
JG531_RS25220 5059983..5060702 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T184923 WP_001173343.1 NZ_CP066753:c5057109-5056936 [Escherichia coli O157:H7]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T184923 NZ_CP087843:c2819948-2819556 [Moraxella bovis]
ATGAAATATTTGCTGGACACCAATGCAGTCATTGCGATTTTGAATGCCAGTGAACGATTTTTGACCACGCTAGAACGGCA
TAAAGAGCGTGAAATTGCCATCTCGTCAATTGTTTTGTCGGAGCTGTATTATGGGGCGTACAAAAGCCAAAAAACCGCCC
AAAACCTTGAAAAAATCAATCTACTACCCTTTGAAGTGTTGCAATTTAATTCACAGGACGCTGATAAGGCTGGCGAAATT
GGTGCAACCCTAGAAAGGCGTGGCACACCCATTGGTTTTTATGACACGCTGATTGCAGGGCAAGCGATTGCCAATGATTT
GATTGTTATTACTGACAATGTGCGAGAATTTCTCCGAGTGGACGGTTTGCAAATTGAAAATTGGTTAAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT184923 WP_000398619.1 NZ_CP066753:c5057433-5057137 [Escherichia coli O157:H7]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT184923 NZ_CP087843:c2820208-2819945 [Moraxella bovis]
ATGCGCCAAACTGCCACCGCAAAATTATTTTGGACTGGCAACAGTCAAGCCGTTCGCTTACCCAAAACCTTTCGTTTTGA
AGGCACGGAAGTGCGTATCAAAAAACAAGGACGACAAGTGATTATTGAGCCAGCAGAGACCCAAGATTGGGCGTGGCTGG
ACAAATTGCGAGCAATGCCAAAAGATGACAGTTTTGAAGAAGCCATTTTGGAAGGTCGCCAAAATTTAAAAGCCGATGAA
CGAGATTGGGGTGCATTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References