Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2892486..2893124 Replicon chromosome
Accession NZ_CP066753
Organism Escherichia coli O157:H7 strain ECP19-598

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag JG531_RS14585 Protein ID WP_000813794.1
Coordinates 2892948..2893124 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag JG531_RS14580 Protein ID WP_001270286.1
Coordinates 2892486..2892902 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG531_RS14560 2887638..2888579 - 942 WP_001251315.1 ABC transporter permease -
JG531_RS14565 2888580..2889593 - 1014 WP_000220402.1 ABC transporter ATP-binding protein -
JG531_RS14570 2889611..2890756 - 1146 WP_000047432.1 ABC transporter substrate-binding protein -
JG531_RS14575 2891001..2892407 - 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
JG531_RS14580 2892486..2892902 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
JG531_RS14585 2892948..2893124 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
JG531_RS14590 2893346..2893576 + 231 WP_000494244.1 YncJ family protein -
JG531_RS14595 2893668..2895629 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
JG531_RS14600 2895702..2896238 - 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
JG531_RS14605 2896291..2897502 + 1212 WP_071525382.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T184912 WP_000813794.1 NZ_CP066753:c2893124-2892948 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T184912 NZ_CP087840:c2090396-2089995 [Moraxella bovis]
ATGTTTAGTTTTATGCTGGATACCAATATCTACATTTATGTGATAAAAGAACGCCCCATAAAAGCATTAAACAAATTTAA
CCAATATGCAGGGCGGATTTGCGTAAGCAGTATCGTGGCAAGCGAGTTGTATTTTGGGGCGTACAACAGCCAATTTATGG
ATAAAAATCTACATCAAGTCGAAGATTTTTTATCACGTTTGACGGTGCTTGATTATACCGCAGAATGCTCGCCCCATTAC
GGCGAGATTTGTGCTGATTTGACCAAGCAGGGCAAGCTCATTAGTGAAAATGACATTCATATCGCCTCTCATGCACGGGC
ATTGGGGCTGACCTTGATTACCAATAATACCGATGAATTTAAACGAGTGTCAGGGCTTAGGATTGGTAATTGGGCGATTT
GA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT184912 WP_001270286.1 NZ_CP066753:c2892902-2892486 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT184912 NZ_CP087840:c2090644-2090411 [Moraxella bovis]
ATGCAAACCAGTGTTTTTTTTAATAATCGCACCCAAGCGGTTCGCATTCCTGCCAAATTTCGCTTTGATGACGATGTCAA
AGCCGTCATGATTAGAAAAGTGGGCAATGAGCGGATTTTGACCCCAATTGATGGTGTGTGGGACAGCTTTTTTTTGAGTG
ATGAGCGAGCGAGTGATGATTTTATGGCAAACCGCCCGACCATGCCAAAAAGCGAGCGATTGACTTTTGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References