Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 907643..908297 Replicon chromosome
Accession NZ_CP066753
Organism Escherichia coli O157:H7 strain ECP19-598

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag JG531_RS04490 Protein ID WP_000244781.1
Coordinates 907890..908297 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JG531_RS04485 Protein ID WP_000354046.1
Coordinates 907643..907909 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG531_RS04465 903731..905164 - 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -
JG531_RS04470 905209..905520 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
JG531_RS04475 905684..906343 + 660 WP_000250274.1 hemolysin III family protein -
JG531_RS04480 906420..907400 - 981 WP_000886053.1 tRNA-modifying protein YgfZ -
JG531_RS04485 907643..907909 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JG531_RS04490 907890..908297 + 408 WP_000244781.1 protein YgfX Toxin
JG531_RS04495 908337..908858 - 522 WP_001055874.1 flavodoxin FldB -
JG531_RS04500 908970..909866 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JG531_RS04505 909891..910601 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JG531_RS04510 910607..912340 + 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T184897 WP_000244781.1 NZ_CP066753:907890-908297 [Escherichia coli O157:H7]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T184897 NZ_CP087837:1758458-1758736 [Moraxella bovis]
ATGATTTTTGAATGGGACGACAACAAAAATCGGACAAACAAGCAAAAGCACGGTGTCAGCTTTGAGAGTGCAAGCCGTAT
TTTTCTTGACCCATTTTTGATTGTTACAGAAGACACAAGTTCTGATGAAGTGCGGTGGCTTGGTGTGGGTAGTGTAGATG
GCGTGGTGATTTTACTCGTTGTACACACCCATACTGATACAGACGGTACAGAAATCATTCGCATTATCTCAGCACGCAAA
CTTAGCAGAGGAGAAATAGAACGCTATGGCTATTGTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT184897 WP_000354046.1 NZ_CP066753:907643-907909 [Escherichia coli O157:H7]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT184897 NZ_CP087837:1758723-1759010 [Moraxella bovis]
ATGGCTATTGTTAGAAAAACCTTAGACGATATCAAACACACACCCATTGATAAAGATGAATTGGCACAACTGCAATCAAT
GAGCGATGATAGCATTAACTACGATGATATTCCGCCATTAACTGACGTTCAAATTGCCAATTTGCGTTTGTACAAGCCCA
TTAAGCAACAAATCACATTAAGGCTTGACAGCGACATTGTGGCGTGGCTTAAAAAAGACGGCAAGGGTTATCAAACTCGT
GCTAATCAATTATTAAGACAGTTAATGTTGAAAGAATTAACCACATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References