Detailed information of TA system TA184893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PsyrTA/-
• Location: 391711..394039
• Replicon: chromosome
• Accession: NZ_CP066753
• Organism: Escherichia coli O157:H7 strain ECP19-598

Toxin (Protein)

• Gene name: PsyrT
• Uniprot ID: A0A6N0MHC8
• Locus tag: JG531_RS01885
• Protein ID: WP_000532146.1
• Coordinates: 391711..392685 (+)
• Length: 325 a.a.

Antitoxin (Protein)

• Gene name: PsyrA
• Uniprot ID: -
• Locus tag: JG531_RS01890
• Protein ID: WP_001303704.1
• Coordinates: 392744..394039 (+)
• Length: 432 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG531_RS01860	387568..388563	+	996	WP_000730263.1	gluconate operon transcriptional repressor GntR	-
JG531_RS01865	388702..389229	+	528	WP_000108330.1	gluconokinase	-
JG531_RS01870	389233..390573	+	1341	WP_000210111.1	gluconate transporter	-
JG531_RS01875	390713..391321	+	609	WP_000410808.1	ATP-dependent DNA helicase	-
JG531_RS01880	391306..391707	+	402	WP_000502498.1	ATP-dependent DNA helicase	-
JG531_RS01885	391711..392685	+	975	WP_000532146.1	ATP-dependent DNA helicase	Toxin
JG531_RS01890	392744..394039	+	1296	WP_001303704.1	DNA-protecting protein DprA	Antitoxin
JG531_RS01895	394085..394678	-	594	WP_001002544.1	NAAT family transporter YhgN	-
JG531_RS01900	394870..395973	+	1104	WP_000799956.1	aspartate-semialdehyde dehydrogenase	-
JG531_RS01905	396246..398432	+	2187	WP_001283734.1	1,4-alpha-glucan branching enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• DNA recombination-mediator protein A (115-429)

Sequences

Toxin

Protein

• Length: 325 a.a.
• Molecular weight: 36460.93 Da
• Isoelectric Point: 9.2667

>T184893 WP_000532146.1 NZ_CP066753:391711-392685 [Escherichia coli O157:H7]
VGRAGRGIDSAVGILLCGSEDRAIHKFFRESAFPAEAQIHEILNVLSVNDGLTLRGIEQRTNLRYGQIEKALKLLVAENP
SPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELADVESYITTKECKMQFLRRALDEPGAEHCGKCSSCLQHPLLSPDI
DSGLLHAANLFIKHADLSLNLNKQVAAGAFTQYGFKGNLPASLQGSTGRILSRWGYSGWGKQVAQEKKTGRFSDELVEAC
AEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFESTSTWFLILPGDWRRNLAYLLLMPLKKSWTIHRRKCSKTVSTSVKIS
TGRL

Nucleotide

• Length: 975 bp

>T184893 NZ_CP087837:c677719-677459 [Moraxella bovis]
ATTGAGGCCTTATTTGTAGCATTAGGGGCGGACATCTCTGAGCGAGCAGGTTCACGCATGGCTGTGGCGCTAAACGGGCA
AGTCATGGTTTTTCATCGCCCACACCCAAGCCCAAATACTGACAAAAGTGCGGTTATGAGCATTAGAAAATGGCTTGATG
GCATGGGTATCACACCATTAGATGACGACAGCCAAACAGACGAATACGCAGACAAACAAACCGACGAACAGGAGCAGACT
CATGAACACAATGACAATTAA

Antitoxin

Protein

• Length: 432 a.a.
• Molecular weight: 47053.95 Da
• Isoelectric Point: 7.2590

>AT184893 WP_001303704.1 NZ_CP066753:392744-394039 [Escherichia coli O157:H7]
MDADSSNSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLLTSDFSRAAASKYKPLSNSEWGKFALWLKHQRISPAE
LLVPQPQEKLTGWSDPRISQERILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLI
AGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENG
ASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESISIERLASCT
GLTIEQISVWLNRAEEEGRVIRLGEGHYQFR

Nucleotide

• Length: 1296 bp

>AT184893 NZ_CP087837:c677478-677143 [Moraxella bovis]
ATGAACACAATGACAATTAACAATCATAAAGCCTTAATCACTTTTGACAGTGAGTTAGGACTGTTTCGTGGGGAGTTTAT
CGGATTAAATGGCGGGGCAGATTTTTATGCTGACAGCGTAGATGGTCTCAATGAACAAGGGGCTTTATCCCTGCAAGCCT
TTTTAGATGTATGTCAAGAACAGGGCATTAGCCCTTATCAGTCGTACAGTGGCAAATTTGTCACACGCCTACCCAAAGAG
TTACATCAAAAAATCGCAGAAACTGCTACCGCCAAGGGTATTAGCCTAAACCAGTGGATTAATGAAACACTCAGCCGTGA
AGTTGGCTTTGCCTGA

Structures

Toxin

• AlphaFold DB: A0A6N0MHC8