Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 367899..368699 Replicon chromosome
Accession NZ_CP066753
Organism Escherichia coli O157:H7 strain ECP19-598

Toxin (Protein)


Gene name ataT Uniprot ID A0A891SNC1
Locus tag JG531_RS01765 Protein ID WP_000342448.1
Coordinates 368172..368699 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag JG531_RS01760 Protein ID WP_001277108.1
Coordinates 367899..368165 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG531_RS01740 363557..364225 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
JG531_RS01745 364218..365276 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
JG531_RS01750 365521..366375 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
JG531_RS01755 366646..367749 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
JG531_RS01760 367899..368165 + 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
JG531_RS01765 368172..368699 + 528 WP_000342448.1 GNAT family N-acetyltransferase Toxin
JG531_RS01770 368696..369079 - 384 WP_000778769.1 aspartate 1-decarboxylase autocleavage activator PanM -
JG531_RS01775 369503..370612 + 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
JG531_RS01780 370660..371586 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
JG531_RS01785 371583..372860 + 1278 WP_000803797.1 branched chain amino acid ABC transporter permease LivM -
JG531_RS01790 372857..373624 + 768 WP_000082097.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19692.68 Da        Isoelectric Point: 7.3232

>T184892 WP_000342448.1 NZ_CP066753:368172-368699 [Escherichia coli O157:H7]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T184892 NZ_CP087837:438703-439095 [Moraxella bovis]
ATGAAATATTTGCTGGACACCAATGCAGTCATTGCGATTTTGAATGCCAGTGAACGATTTTTGACCACGCTAGAACGGCA
TAAAGAGCGTGAAATTGCCATCTCGTCAATTGTTTTGTCGGAGCTGTATTATGGGGCGTACAAAAGCCAAAAAACCGCCC
AAAACCTTGAAAAAATCAATCTACTACCCTTTGAAGTGTTGCAATTTAATTCACAGGACGCTGATAAGGCTGGCGAAATT
GGTGCAACCCTAGAAAGGCGTGGCACACCCATTGGTTTTTATGACACGCTGATTGCAGGGCAAGCGATTGCCAATGATTT
GATTGTTATTACTGACAATGTGCGAGAATTTCTCCGAGTGGACGGTTTGCAAATTGAAAATTGGTTAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT184892 WP_001277108.1 NZ_CP066753:367899-368165 [Escherichia coli O157:H7]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT184892 NZ_CP087837:438443-438706 [Moraxella bovis]
ATGCGCCAAACTGCCACCGCAAAATTATTTTGGACTGGCAACAGTCAAGCCGTTCGCTTACCCAAAGCCTTTCGTTTTGA
AGGCACGGAAGTGCGTATCAAAAAACAAGGACGACAAGTGATTATTGAGCCAGCAGAGACCCAAGATTGGGCGTGGCTGG
ACAAATTGCGAGCAATGCCAAAAGATGACAGTTTTGAAGAAGCCATTTTGGAAGGTCGCCAAAATTTAAAAGCCGATGAA
CGAGATTGGGGTGCATTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6GTS

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References