Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 325468..326099 Replicon chromosome
Accession NZ_CP066753
Organism Escherichia coli O157:H7 strain ECP19-598

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag JG531_RS01515 Protein ID WP_001259386.1
Coordinates 325468..325743 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag JG531_RS01520 Protein ID WP_000593555.1
Coordinates 325740..326099 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG531_RS01500 320472..321539 + 1068 WP_000361458.1 HlyD family secretion protein -
JG531_RS01505 321536..324271 + 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
JG531_RS01510 324271..325395 + 1125 WP_001301659.1 ABC transporter permease -
JG531_RS01515 325468..325743 + 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
JG531_RS01520 325740..326099 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JG531_RS01525 326149..327009 - 861 WP_001131788.1 class II aldolase -
JG531_RS01530 327041..327310 - 270 WP_000933060.1 HPr family phosphocarrier protein -
JG531_RS01535 327300..328808 - 1509 WP_001301773.1 carbohydrate kinase -
JG531_RS01540 328801..330159 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
JG531_RS01545 330236..330517 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
JG531_RS01550 330514..330987 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 316612..326099 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T184891 WP_001259386.1 NZ_CP066753:325468-325743 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T184891 NZ_CP087837:435492-435764 [Moraxella bovis]
GTGGCTAAACGTAGTTTTGAAATGGGCAATCCCTTTAAAAGGGATTTAAAAAAGCATTATTTGGCATTGATTGGCGAAGC
GTGGACGGAAGTGTCTGATTGCTTGTTAAATGCCAAACCCATACCTCAAAAATACCAAGACCACCCATTGCAAGGCGATT
GGGTGGGGTTTAGAGATTGTCATATCAAGCCTGATTTGGTGCTAATTTATAAAATAAATGATGAAGCGGTGGAATTACAT
CGGCTTGCCACGCATAGCGAGTTGTTTGGTTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT184891 WP_000593555.1 NZ_CP066753:325740-326099 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT184891 NZ_CP087837:435206-435499 [Moraxella bovis]
ATGAGCCACACCAATTTTAATATGCGATTAGACAACGACCTACGCCAAAAAGCCTATCCTGTGCTAGAACAATACGGCTT
GACTCCAAGCCAAGCAGTGCGGATGTTTTTTAATCAAATCGCCCAAACAGGCAAAGTGCCATTGTCTTTTGATTGGGGGC
AGTCGCATACGCTTAGTAGCAATGGCGAAAAGCTGTTGCGTGAGAGTATTCAAGCTTTTGAAAATGGCGAAACCGAGCGA
TTTGATAGCTTAGATGAGTTAAATCAAGCAATGGCGGAGATTGCTCGTGGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References