Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 59077..59602 Replicon plasmid unnamed2
Accession NZ_CP066750
Organism Escherichia coli O157:H7 strain ECP19-2498

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag JG532_RS28375 Protein ID WP_001159868.1
Coordinates 59077..59382 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag JG532_RS28380 Protein ID WP_000813634.1
Coordinates 59384..59602 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG532_RS28360 55503..56258 - 756 WP_000852148.1 replication initiation protein RepE -
JG532_RS28365 56334..57547 + 1214 WP_162829202.1 IS3 family transposase -
JG532_RS28370 58270..59076 - 807 WP_000016989.1 site-specific integrase -
JG532_RS28375 59077..59382 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
JG532_RS28380 59384..59602 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JG532_RS28385 60061..60744 + 684 WP_010891291.1 DNA-binding protein -
JG532_RS28390 60741..61361 + 621 WP_001248529.1 hypothetical protein -
JG532_RS28395 61358..61558 + 201 WP_000708307.1 hypothetical protein -
JG532_RS28400 61966..62943 + 978 WP_000361615.1 RepB family plasmid replication initiator protein -
JG532_RS28405 63228..63968 - 741 WP_001066920.1 tyrosine-type recombinase/integrase -
JG532_RS28410 64089..64319 - 231 WP_010891288.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / toxB / hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE 1..95598 95598


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T184890 WP_001159868.1 NZ_CP066750:c59382-59077 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T184890 NZ_CP087837:c100931-100596 [Moraxella bovis]
ATGTTGCATTTTGCTGAAACATCATTATTCACTAAACAAATCATAGACTTAATGGATGATGACGAGTACAGACTATTACA
AACAAACTTGATGAAAAACCCACATCAAGGCGACATTGTTCGTGGTACTGGCGGAGTACGCAAAACAAGATGGTCAATTG
ATGGCAAGGGCAAAAGTGGTGGCAGTCGCATCATTTACTTTTTTGTTGATGGTGCTGGCATTTTCTTTATGCTCTTGGCT
TATCCTAAATCAAAACAAACAACGCTATCGGCGGATGAGAAAAAAGAAATGCTCAAACTAACCACCGCAATTAAGGAGAT
TTACCGTGCAAAATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT184890 WP_000813634.1 NZ_CP066750:c59602-59384 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT184890 NZ_CP087837:c100606-100325 [Moraxella bovis]
GTGCAAAATGAGTTTTACGATGATTTAAAAGCCTCATTGACTGAGGCTCTGGCAATCGCTAAGGGCGAAATTTCCCCAAG
TCGTACATTCACTTATGAACGCCCAAACATCAAAGATATTCGGGCAAAAACAGGGCTATCACAAACCCAGTTCGCCCAGA
AGCTACACATAAGCCCTAAAACGCTTAAAAACTGGGAACAGGGCATTAGAACGCCAACAGGTCCAGCCATTACCCTAATT
AGATTACTTGACAAAAACCCTGATTTAATTAGCATGGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References