Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 12963..13522 | Replicon | plasmid unnamed2 |
| Accession | NZ_CP066750 | ||
| Organism | Escherichia coli O157:H7 strain ECP19-2498 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | O82928 |
| Locus tag | JG532_RS28115 | Protein ID | WP_000421248.1 |
| Coordinates | 12963..13238 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | C1J8K5 |
| Locus tag | JG532_RS28120 | Protein ID | WP_001178089.1 |
| Coordinates | 13238..13522 (-) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JG532_RS28080 | 8316..8705 | - | 390 | WP_001172748.1 | cytochrome b562 family protein | - |
| JG532_RS28085 | 8749..10959 | - | 2211 | WP_000592771.1 | catalase/peroxidase KatP | - |
| JG532_RS28090 | 11136..11321 | + | 186 | WP_001302179.1 | hypothetical protein | - |
| JG532_RS28095 | 11321..11737 | + | 417 | Protein_10 | IS91 family transposase | - |
| JG532_RS28100 | 11737..12303 | + | 567 | Protein_11 | IS3-like element IS600 family transposase | - |
| JG532_RS28105 | 12347..12562 | + | 216 | Protein_12 | transposase | - |
| JG532_RS28110 | 12662..12868 | - | 207 | WP_001105064.1 | hypothetical protein | - |
| JG532_RS28115 | 12963..13238 | - | 276 | WP_000421248.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| JG532_RS28120 | 13238..13522 | - | 285 | WP_001178089.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| JG532_RS28125 | 14434..15291 | - | 858 | WP_000130945.1 | incFII family plasmid replication initiator RepA | - |
| JG532_RS28130 | 15284..15358 | - | 75 | WP_001370046.1 | RepA leader peptide Tap | - |
| JG532_RS28135 | 15594..15848 | - | 255 | WP_000083831.1 | replication regulatory protein RepA | - |
| JG532_RS28140 | 16088..16426 | - | 339 | WP_000766796.1 | hypothetical protein | - |
| JG532_RS28145 | 16464..16673 | - | 210 | WP_001302200.1 | hemolysin expression modulator Hha | - |
| JG532_RS28150 | 16719..17180 | - | 462 | WP_001233853.1 | thermonuclease family protein | - |
| JG532_RS28155 | 17425..17637 | - | 213 | WP_001302189.1 | ANR family transcriptional regulator | - |
| JG532_RS28160 | 17770..18330 | - | 561 | WP_000840472.1 | fertility inhibition protein FinO | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | espP / toxB / hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE | 1..95598 | 95598 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10735.42 Da Isoelectric Point: 9.9324
>T184889 WP_000421248.1 NZ_CP066750:c13238-12963 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T184889 NZ_CP087831:c53301-53110 [Moraxella bovis]
ATGGCAAGCAGTAGAGAAGTCATTAAAATCATAAAAGCTGACGGTTGGTATCGTGTTAAATCAAATGGCGGTAGTCATCA
GCAATTCAAACACCCCACCAAAAAAGGTCGAGTTACTGTTCCCCACCCCAAAAAAGACTTAGCACAACCCACCATTGACA
GCATTATGAGACAAGCAGGGCTAAGCGATTAG
ATGGCAAGCAGTAGAGAAGTCATTAAAATCATAAAAGCTGACGGTTGGTATCGTGTTAAATCAAATGGCGGTAGTCATCA
GCAATTCAAACACCCCACCAAAAAAGGTCGAGTTACTGTTCCCCACCCCAAAAAAGACTTAGCACAACCCACCATTGACA
GCATTATGAGACAAGCAGGGCTAAGCGATTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10474.89 Da Isoelectric Point: 5.6558
>AT184889 WP_001178089.1 NZ_CP066750:c13522-13238 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
Download Length: 285 bp
>AT184889 NZ_CP087831:c53076-52672 [Moraxella bovis]
ATGTTATACCCCATTGCAATCTTAAAGGGCGATGATAAAACCGCCCACGGTATCTTTTTTCCCGATGTGCCAAATCTTGC
CAGTGCGTGCGATGACTTGCAAGACGTACACAAAATGGCACTAGAATGCCTTGATGTGCATTTTAGTGGCTTGGTAGAAG
ACGGCGAAGAAATCCCATTACCCACCAATTTTGACGCACACACCCAAAATAGCCAATTTGACGGCATGATGTGGGCGTGG
GTAGATGTGGACTTGTCAAAATATGACGTAAAAAGCCATAAAATCAACATTACCTTGCCTAACTATCTGATTGCCAAAAT
TGACGAAAAAGTCTTGTCTCATAAATCGCTATACAAAAGCCGTTCTAATTATTTGGCACAATTGGCGATGGCAGATTTGG
GGTAG
ATGTTATACCCCATTGCAATCTTAAAGGGCGATGATAAAACCGCCCACGGTATCTTTTTTCCCGATGTGCCAAATCTTGC
CAGTGCGTGCGATGACTTGCAAGACGTACACAAAATGGCACTAGAATGCCTTGATGTGCATTTTAGTGGCTTGGTAGAAG
ACGGCGAAGAAATCCCATTACCCACCAATTTTGACGCACACACCCAAAATAGCCAATTTGACGGCATGATGTGGGCGTGG
GTAGATGTGGACTTGTCAAAATATGACGTAAAAAGCCATAAAATCAACATTACCTTGCCTAACTATCTGATTGCCAAAAT
TGACGAAAAAGTCTTGTCTCATAAATCGCTATACAAAAGCCGTTCTAATTATTTGGCACAATTGGCGATGGCAGATTTGG
GGTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z6FAZ0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A731IHA8 |