Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 70363..70922 Replicon plasmid unnamed1
Accession NZ_CP066749
Organism Escherichia coli O157:H7 strain ECP19-2498

Toxin (Protein)


Gene name relE Uniprot ID A0A733EV64
Locus tag JG532_RS27905 Protein ID WP_001582571.1
Coordinates 70363..70638 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag JG532_RS27910 Protein ID WP_001178089.1
Coordinates 70638..70922 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG532_RS27875 65723..66325 + 603 WP_000517695.1 hypothetical protein -
JG532_RS27880 66381..66875 + 495 WP_001582574.1 hypothetical protein -
JG532_RS27885 66875..67141 + 267 WP_000627829.1 hypothetical protein -
JG532_RS27890 67336..67614 - 279 WP_001105069.1 hypothetical protein -
JG532_RS27895 67923..69458 + 1536 WP_023181050.1 IS21 family transposase -
JG532_RS27900 69475..70230 + 756 WP_001282653.1 IS21-like element ISSso4 family helper ATPase IstB -
JG532_RS27905 70363..70638 - 276 WP_001582571.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JG532_RS27910 70638..70922 - 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
JG532_RS27915 71830..72861 - 1032 WP_000907861.1 replication initiation protein -
JG532_RS27920 73834..74097 + 264 WP_001324596.1 ash family protein -
JG532_RS27925 74015..74302 + 288 WP_000074849.1 conjugal transfer protein TraA -
JG532_RS27930 74744..75277 + 534 WP_001306770.1 transcription termination factor NusG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B - 1..95679 95679


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10807.48 Da        Isoelectric Point: 9.5296

>T184888 WP_001582571.1 NZ_CP066749:c70638-70363 [Escherichia coli O157:H7]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNDQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T184888 NZ_CP087830:2851949-2852284 [Moraxella bovis]
ATGTTGCATTTTGCTGAAACATCATTATTCACTAAACAAATCATAGACTTAATGGATGATGACGAGTACAGACTATTACA
AACAAACTTGATGAAAAACCCACATCAAGGCGACATTGTTCGTGGTACTGGCGGAGTACGCAAAACAAGATGGTCAATTG
ATGGCAAGGGCAAAAGTGGTGGCAGTCGCATCATTTACTTTTTTGTTGATGGTGCTGGCATTTTCTTTATGCTCTTGGCT
TATCCTAAATCAAAACAAACAACGCTATCGGCGGATGAGAAAAAAGAAATGCTCAAACTAACCACCGCAATTAAGGAGAT
TTACCGTGCAAAATGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT184888 WP_001178089.1 NZ_CP066749:c70922-70638 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT184888 NZ_CP087830:2852274-2852555 [Moraxella bovis]
GTGCAAAATGAGTTTTACGATGATTTAAAAGCCTCATTGACTGAGGCTCTGGCAATCGCTAAGGGCGAAATTTCCCCAAG
TCGTACATTCACTTATGAACGCCCAAACATCAAAGATATTCGGGCAAAAACAGGGCTATCACAAACCCAGTTCGCCCAGA
AGCTACACATAAGCCCTAAAACGCTTAAAAACTGGGAACAGGGCATTAGAACGCCAACAGGTCCAGCCATTACCCTAATT
AGATTACTTGACAAAAACCCTGATTTAATTAGCATGGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A733EV64

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References