Detailed information of TA system TA184882

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4917617..4918212
• Replicon: chromosome
• Accession: NZ_CP066748
• Organism: Escherichia coli O157:H7 strain ECP19-2498

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: JG532_RS24645
• Protein ID: WP_000239579.1
• Coordinates: 4917617..4917967 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: JG532_RS24650
• Protein ID: WP_001223210.1
• Coordinates: 4917961..4918212 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG532_RS24625	4913063..4914085	-	1023	WP_001301928.1	ABC transporter permease	-
JG532_RS24630	4914099..4915601	-	1503	WP_000205805.1	sugar ABC transporter ATP-binding protein	-
JG532_RS24635	4915741..4916697	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
JG532_RS24640	4917007..4917537	+	531	WP_000055075.1	inorganic diphosphatase	-
JG532_RS24645	4917617..4917967	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
JG532_RS24650	4917961..4918212	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
JG532_RS24655	4918424..4918765	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
JG532_RS24660	4918768..4922547	-	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T184882 WP_000239579.1 NZ_CP066748:c4917967-4917617 [Escherichia coli O157:H7]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T184882 NZ_CP087830:1249932-1250174 [Moraxella bovis]
ATGAATTATAGCGAATTTCGCCAAAACCTAGCGTCCGCCCTTGATTATGTTCAAGACAGCCATGCCCCTGTGATTGTCAA
ACGTGGCAAACATTCTGCGGTGGTGATTAGTCTTGATGAATATAATGCTTTTAAAGAAACAGATTATCTATTGTCAAACC
CTGCTAATGCCGAACACCTTATGCGTGGCGTGCAAGCGGTAAAAAATCGTCAACTTACCCAAAGGGATTTGATTGAGGAT
TAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT184882 WP_001223210.1 NZ_CP066748:c4918212-4917961 [Escherichia coli O157:H7]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT184882 NZ_CP087830:1250164-1250421 [Moraxella bovis]
TTGAGGATTAGATGGTTTGATGAGGTATGGGAGGATTATTTGTATTGGCAAAGCCAAGATAAGAAAACCATAAAACGTAT
TAATACCTTGATTAAGGATTGCAGACGAGACCCTTTTGACGGTATCGGCAAACCCGAACCATTAAAATATAACCTAACAG
GCTACTGGTTAAGACGCATTGATGATGCCAACCGCTTGGTTTATTGTTGCGAAAGTGAAACACTCATCATTTCATGCCGT
CATCATTATGAGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8