Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4150988..4151606 Replicon chromosome
Accession NZ_CP066748
Organism Escherichia coli O157:H7 strain ECP19-2498

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JG532_RS21085 Protein ID WP_001291435.1
Coordinates 4151388..4151606 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JG532_RS21080 Protein ID WP_000344800.1
Coordinates 4150988..4151362 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG532_RS21070 4146077..4147270 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JG532_RS21075 4147293..4150442 + 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
JG532_RS21080 4150988..4151362 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JG532_RS21085 4151388..4151606 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
JG532_RS21090 4151778..4152329 + 552 WP_000102553.1 maltose O-acetyltransferase -
JG532_RS21095 4152445..4152915 + 471 WP_000136192.1 YlaC family protein -
JG532_RS21100 4153079..4154629 + 1551 WP_001302952.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JG532_RS21105 4154671..4155024 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JG532_RS21115 4155403..4155714 + 312 WP_000409911.1 MGMT family protein -
JG532_RS21120 4155747..4156319 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T184876 WP_001291435.1 NZ_CP066748:4151388-4151606 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T184876 NZ_CP087828:c2108692-2108291 [Moraxella bovis]
ATGTTTAGTTTTATGCTGGATACCAATATCTACATTTATGTGATAAAAGAACGCCCCATAAAAGCATTAAACAAATTTAA
CCAATATGCAGGGCGGATTTGCGTAAGCAGTATCGTGGCAAGCGAGTTGTATTTTGGGGCGTACAACAGCCAATTTATGG
ATAAAAATCTACATCAAGTCGAAGATTTTTTATCACGTTTGACGGTGCTTGATTATACCGCAGAATGCTCGCCCCATTAC
GGCGAGATTTGTGCTGATTTGACCAAGCAGGGCAAGCTCATTAGTGAAAATGACATTCATATCGCCTCTCATGCACGGGC
ATTGGGGCTGACCTTGATTACCAATAATACCGATGAATTTAAACGAGTGTCAGGGCTTAGGATTGGTAATTGGGCGATTT
GA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT184876 WP_000344800.1 NZ_CP066748:4150988-4151362 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT184876 NZ_CP087828:c2108940-2108707 [Moraxella bovis]
ATGCAAACCAGTGTTTTTTTTAATAATCGCACCCAAGCGGTTCGCATTCCTGCCAAATTTCGCTTTGATGACGATGTCAA
AGCCGTCATGATTAGAAAAGTGGGCAATGAGCGGATTTTGACCCCAATTGATGGTGTGTGGGACAGCTTTTTTTTGAGTG
ATGAGCGAGCGAGTGATGATTTTATGGCAAACCGCCCGACCATGCCAAAAAGCGAGCGATTGACTTTTGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References