Detailed information of TA system TA184874

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3566086..3566881
• Replicon: chromosome
• Accession: NZ_CP066748
• Organism: Escherichia coli O157:H7 strain ECP19-2498

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: JG532_RS18320
• Protein ID: WP_000854914.1
• Coordinates: 3566086..3566460 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JG532_RS18325
• Protein ID: WP_001416641.1
• Coordinates: 3566507..3566881 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG532_RS18290	3562764..3563468	+	705	WP_001241680.1	leucyl/phenylalanyl-tRNA--protein transferase	-
JG532_RS18295	3563753..3563971	+	219	WP_001040187.1	translation initiation factor IF-1	-
JG532_RS18305	3564462..3565304	-	843	WP_001280481.1	DUF4942 domain-containing protein	-
JG532_RS18310	3565389..3565586	-	198	WP_000839282.1	DUF957 domain-containing protein	-
JG532_RS18315	3565598..3566089	-	492	WP_000976853.1	hypothetical protein	-
JG532_RS18320	3566086..3566460	-	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
JG532_RS18325	3566507..3566881	-	375	WP_001416641.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JG532_RS18330	3567044..3567265	-	222	WP_001220314.1	DUF987 domain-containing protein	-
JG532_RS18335	3567328..3567804	-	477	WP_001186738.1	RadC family protein	-
JG532_RS18340	3567820..3568305	-	486	WP_000214398.1	antirestriction protein	-
JG532_RS18345	3568396..3569214	-	819	WP_001234682.1	DUF945 domain-containing protein	-
JG532_RS18350	3569554..3570624	-	1071	WP_000102669.1	patatin-like phospholipase family protein	-
JG532_RS18355	3570621..3571526	-	906	WP_000203541.1	chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T184874 WP_000854914.1 NZ_CP066748:c3566460-3566086 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T184874 NZ_CP087828:1821424-1821702 [Moraxella bovis]
ATGATTCAAAGTTTTAAGTGCAAAGATGCCCAAAGCCTGTTTGAAACAGGGTCAAGCCGTGTATTTGCCAACTTTTTATC
CGTTGCCACTCGCAAACTTACCATGCTTGATAATGCCGTGGCACTTGCCGACCTACGCATTCCACCTGCCAACCGCCTTG
AAAAGCTGACAGGCGATAGAGAAGGTCAGTATAGTATTCGCATTAACGACCAATTTCGCATTTGCTTTGTGTGGGGCGAT
GATGGCGTGTATGATGTTGAGATTGTTGATTATCACTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13778.61 Da
• Isoelectric Point: 6.6249

>AT184874 WP_001416641.1 NZ_CP066748:c3566881-3566507 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGFCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT184874 NZ_CP087828:1821717-1822022 [Moraxella bovis]
ATGTTCAAAAATGGCATGAGACCTGTTCACGCAGGTGAGGTATTGCGTGAAGAATATCTAATCCCAATGGGTATGTCCGC
CACCGCCTTAGCAAATGCATTGGGCGTAACCCCTGCACGCATTAACGACATCGTGCGGCAGCGGCGTGGCGTTACGGCTG
ATACCGCTTTGCGTTTGGCTCGTTATTTTGGCGGTACGGCACAATTTTGGCTAAACTTACAAACCACCTATGAGCTTCGC
CTTGCCGAAACTACGCTTGGCGACAGTTTGGATTTTATCAGCCCTCATGAAGTTGCCCATGCCTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5