Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2776772..2777410 Replicon chromosome
Accession NZ_CP066748
Organism Escherichia coli O157:H7 strain ECP19-2498

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag JG532_RS14055 Protein ID WP_000813794.1
Coordinates 2776772..2776948 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag JG532_RS14060 Protein ID WP_001270286.1
Coordinates 2776994..2777410 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG532_RS14035 2772394..2773605 - 1212 WP_071525382.1 BenE family transporter YdcO -
JG532_RS14040 2773658..2774194 + 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
JG532_RS14045 2774267..2776228 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
JG532_RS14050 2776320..2776550 - 231 WP_000494244.1 YncJ family protein -
JG532_RS14055 2776772..2776948 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
JG532_RS14060 2776994..2777410 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
JG532_RS14065 2777489..2778895 + 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
JG532_RS14070 2779140..2780285 + 1146 WP_000047432.1 ABC transporter substrate-binding protein -
JG532_RS14075 2780303..2781316 + 1014 WP_000220402.1 ABC transporter ATP-binding protein -
JG532_RS14080 2781317..2782258 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T184868 WP_000813794.1 NZ_CP066748:2776772-2776948 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T184868 NZ_CP087828:c100843-100508 [Moraxella bovis]
ATGTTGCATTTTGCTGAAACATCATTATTCACTAAACAAATCATAGACTTAATGGATGATGACGAGTACAGACTATTACA
AACAAACTTGATGAAAAACCCACATCAAGGCGACATTGTTCGTGGTACTGGCGGAGTACGCAAAACAAGATGGTCAATTG
ATGGCAAGGGCAAAAGTGGTGGCAGTCGCATCATTTACTTTTTTGTTGATGGTGCTGGCATTTTCTTTATGCTCTTGGCT
TATCCTAAATCAAAACAAACAACGCTATCGGCGGATGAGAAAAAAGAAATGCTCAAACTAACCACCGCAATTAAGGAGAT
TTACCGTGCAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT184868 WP_001270286.1 NZ_CP066748:2776994-2777410 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT184868 NZ_CP087828:c100518-100237 [Moraxella bovis]
GTGCAAAATGAGTTTTACGATGATTTAAAAGCCTCATTGACTGAGGCTCTGGCAATCGCTAAGGGCGAAATTTCCCCAAG
TCGTACATTCACTTATGAACGCCCAAACATCAAAGATATTCGGGCAAAAACAGGGCTATCACAAACCCAGTTCGCCCAGA
AGCTACACATAAGCCCTAAAACGCTTAAAAACTGGGAACAGGGCATTAGAACGCCAACAGGTCCAGCCATTACCCTAATT
AGATTACTTGACAAAAACCCTGATTTAATTAGCATGGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References