Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 896356..897010 Replicon chromosome
Accession NZ_CP066748
Organism Escherichia coli O157:H7 strain ECP19-2498

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag JG532_RS04405 Protein ID WP_000244781.1
Coordinates 896603..897010 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JG532_RS04400 Protein ID WP_000354046.1
Coordinates 896356..896622 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG532_RS04380 892444..893877 - 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -
JG532_RS04385 893922..894233 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
JG532_RS04390 894397..895056 + 660 WP_000250274.1 hemolysin III family protein -
JG532_RS04395 895133..896113 - 981 WP_000886053.1 tRNA-modifying protein YgfZ -
JG532_RS04400 896356..896622 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JG532_RS04405 896603..897010 + 408 WP_000244781.1 protein YgfX Toxin
JG532_RS04410 897050..897571 - 522 WP_001055874.1 flavodoxin FldB -
JG532_RS04415 897683..898579 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JG532_RS04420 898604..899314 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JG532_RS04425 899320..901053 + 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T184857 WP_000244781.1 NZ_CP066748:896603-897010 [Escherichia coli O157:H7]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T184857 NZ_CP087825:966548-966730 [Moraxella bovis]
ATGAAGTATTCAGAGTTTCAAAGGTGGCTATTGGCACAAGGGGCAACGGTAAACAAGCAAGGCGGTAAAGGCTCGCACCG
TAAAGTTACCCTAAATGGCAAAACCACCACTTTCCCTTATCACGGCAGTAAAGAGATTGGGGAAGGACTAAGAAAGAAAA
TTTTAAAAGACTTGGATTTATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT184857 WP_000354046.1 NZ_CP066748:896356-896622 [Escherichia coli O157:H7]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT184857 NZ_CP087825:966792-967205 [Moraxella bovis]
ATGTACTATCCTGCTACTTTTACCCCTGATACCAATGATACTTTTTTGGTGGCGTTTCGTGATATTCCCGAAGCGGTGGG
CGTGGGTGAAACCTTTGAAATCGCCTACCAATCCGCCCTTGACGGCTTAGAAACGGCATTTAGCATCTATATGGACGAAC
GCAAGCCAATCCCCGCCCCCAGTGAGCTGTCTGACGGCGAACACGCCATTTATTTGCCTGTGGCGGTACAGACCAAACTT
GCCTTATATCACGAAATGCTAGCCCAAGGCGTTACTAAGGCGGAGCTGGCTCGCCGTCTGTCTGTCAATCAAAAACAAAT
TGATAGACTTTGGGACGTGAGCCACTCCACCAAATTAGAATTTTTGGAAAAGGCGTTTAGCGTGCTAGGCAAACGCCTAT
CCCTAGCCATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References