Detailed information of TA system TA184853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PsyrTA/-
• Location: 381743..384071
• Replicon: chromosome
• Accession: NZ_CP066748
• Organism: Escherichia coli O157:H7 strain ECP19-2498

Toxin (Protein)

• Gene name: PsyrT
• Uniprot ID: A0A6N0MHC8
• Locus tag: JG532_RS01815
• Protein ID: WP_000532146.1
• Coordinates: 381743..382717 (+)
• Length: 325 a.a.

Antitoxin (Protein)

• Gene name: PsyrA
• Uniprot ID: -
• Locus tag: JG532_RS01820
• Protein ID: WP_001303704.1
• Coordinates: 382776..384071 (+)
• Length: 432 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG532_RS01790	377600..378595	+	996	WP_000730263.1	gluconate operon transcriptional repressor GntR	-
JG532_RS01795	378734..379261	+	528	WP_000108330.1	gluconokinase	-
JG532_RS01800	379265..380605	+	1341	WP_000210111.1	gluconate transporter	-
JG532_RS01805	380745..381353	+	609	WP_000410808.1	ATP-dependent DNA helicase	-
JG532_RS01810	381338..381739	+	402	WP_000502498.1	ATP-dependent DNA helicase	-
JG532_RS01815	381743..382717	+	975	WP_000532146.1	ATP-dependent DNA helicase	Toxin
JG532_RS01820	382776..384071	+	1296	WP_001303704.1	DNA-protecting protein DprA	Antitoxin
JG532_RS01825	384117..384710	-	594	WP_001002544.1	NAAT family transporter YhgN	-
JG532_RS01830	384902..386005	+	1104	WP_000799956.1	aspartate-semialdehyde dehydrogenase	-
JG532_RS01835	386278..388464	+	2187	WP_001283704.1	1,4-alpha-glucan branching enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• DNA recombination-mediator protein A (115-429)

Sequences

Toxin

Protein

• Length: 325 a.a.
• Molecular weight: 36460.93 Da
• Isoelectric Point: 9.2667

>T184853 WP_000532146.1 NZ_CP066748:381743-382717 [Escherichia coli O157:H7]
VGRAGRGIDSAVGILLCGSEDRAIHKFFRESAFPAEAQIHEILNVLSVNDGLTLRGIEQRTNLRYGQIEKALKLLVAENP
SPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELADVESYITTKECKMQFLRRALDEPGAEHCGKCSSCLQHPLLSPDI
DSGLLHAANLFIKHADLSLNLNKQVAAGAFTQYGFKGNLPASLQGSTGRILSRWGYSGWGKQVAQEKKTGRFSDELVEAC
AEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFESTSTWFLILPGDWRRNLAYLLLMPLKKSWTIHRRKCSKTVSTSVKIS
TGRL

Nucleotide

• Length: 975 bp

>T184853 NZ_CP087823:c2406239-2405847 [Moraxella bovis]
ATGAAATATTTGCTGGACACCAATGCAGTCATTGCGATTTTGAATGCCAGTGAACGATTTTTGACCACGCTAGAACGGCA
TAAAGAGCGTGAAATTGCCATCTCGTCAATTGTTTTGTCGGAGCTGTATTATGGGGCGTACAAAAGCCAAAAAACCGCCC
AAAACCTTGAAAAAATCAATCTACTACCCTTTGAAGTGTTGCAATTTAATTCACAGGACGCTGATAAGGCTGGCGAAATT
GGTGCAACCCTAGAAAGGCGTGGCACACCCATTGGTTTTTATGACACGCTGATTGCAGGGCAAGCGATTGCCAATGATTT
GATTGTTATTACTGACAATGTGCGAGAATTTCTCCGAGTGGACGGTTTGCAAATTGAAAATTGGTTAAAATAA

Antitoxin

Protein

• Length: 432 a.a.
• Molecular weight: 47053.95 Da
• Isoelectric Point: 7.2590

>AT184853 WP_001303704.1 NZ_CP066748:382776-384071 [Escherichia coli O157:H7]
MDADSSNSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLLTSDFSRAAASKYKPLSNSEWGKFALWLKHQRISPAE
LLVPQPQEKLTGWSDPRISQERILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLI
AGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENG
ASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESISIERLASCT
GLTIEQISVWLNRAEEEGRVIRLGEGHYQFR

Nucleotide

• Length: 1296 bp

>AT184853 NZ_CP087823:c2406499-2406236 [Moraxella bovis]
ATGCGCCAAACTGCCACCGCAAAATTATTTTGGACTGGCAACAGTCAAGCCGTTCGCTTACCCAAAACCTTTCGTTTTGA
AGGCACGGAAGTGCGTATCAAAAAACAAGGACGACAAGTGATTATTGAGCCAGCAGAGACCCAAGATTGGGCGTGGCTGG
ACAAATTGCGAGCAATGCCAAAAGATGACAGTTTTGAAGAAGCCATTTTGGAAGGTCGCCAAAATTTAAAAGCCGATGAA
CGAGATTGGGGTGCATTTGAATGA

Structures

Toxin

• AlphaFold DB: A0A6N0MHC8