Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 357932..358732 Replicon chromosome
Accession NZ_CP066748
Organism Escherichia coli O157:H7 strain ECP19-2498

Toxin (Protein)


Gene name ataT Uniprot ID A0A891SNC1
Locus tag JG532_RS01695 Protein ID WP_000342448.1
Coordinates 358205..358732 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag JG532_RS01690 Protein ID WP_001277108.1
Coordinates 357932..358198 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG532_RS01670 353590..354258 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
JG532_RS01675 354251..355309 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
JG532_RS01680 355554..356408 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
JG532_RS01685 356679..357782 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
JG532_RS01690 357932..358198 + 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
JG532_RS01695 358205..358732 + 528 WP_000342448.1 GNAT family N-acetyltransferase Toxin
JG532_RS01700 358729..359112 - 384 WP_000778769.1 aspartate 1-decarboxylase autocleavage activator PanM -
JG532_RS01705 359536..360645 + 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
JG532_RS01710 360693..361619 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
JG532_RS01715 361616..362893 + 1278 WP_000803797.1 branched chain amino acid ABC transporter permease LivM -
JG532_RS01720 362890..363657 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19692.68 Da        Isoelectric Point: 7.3232

>T184852 WP_000342448.1 NZ_CP066748:358205-358732 [Escherichia coli O157:H7]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T184852 NZ_CP087823:1267372-1267629 [Moraxella bovis]
TTGAGGATTAGATGGTTTGATGAGGTATGGGAGGATTATTTGTATTGGCAAAGCCAAGATAAGAAAACCATAAAACGTAT
TAATACCTTGATTAAGGATTGCAGACGAGACCCTTTTGACGGTATCGGCAAACCCGAACCATTAAAATATAACCTAACAG
GCTACTGGTTAAGACGCATTAATGATGCCAACCGCTTGGTTTATTGTTGCGAAAGTGAAACACTCATCATTTCATGCCGT
CATCATTATGAGGCTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT184852 WP_001277108.1 NZ_CP066748:357932-358198 [Escherichia coli O157:H7]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT184852 NZ_CP087823:1267140-1267382 [Moraxella bovis]
ATGAATTATAGCGAATTTCGCCAAAACCTAGCCTCTGCCCTTGATTATGTTCAAGACAGCCATGCCCCTGTGATTGTCAA
ACGTGGCAAACATTCTGCGGTGGTGATTAGTCTTGATGAATATAATGCTTTTAAAGAAACAGATTATCTATTGTCAAACC
CTGCTAATGCCGAACACCTTATGCGTGGCGTGCAAGCGGTAAAAAATCGTCAACTTACCCAAAGGGATTTGATTGAGGAT
TAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6GTS

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References