Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 315501..316132 Replicon chromosome
Accession NZ_CP066748
Organism Escherichia coli O157:H7 strain ECP19-2498

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag JG532_RS01445 Protein ID WP_001259385.1
Coordinates 315501..315776 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag JG532_RS01450 Protein ID WP_000593555.1
Coordinates 315773..316132 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG532_RS01430 310505..311572 + 1068 WP_000361458.1 HlyD family secretion protein -
JG532_RS01435 311569..314304 + 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
JG532_RS01440 314304..315428 + 1125 WP_001301659.1 ABC transporter permease -
JG532_RS01445 315501..315776 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
JG532_RS01450 315773..316132 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JG532_RS01455 316182..317042 - 861 WP_001131788.1 class II aldolase -
JG532_RS01460 317074..317343 - 270 WP_000933060.1 HPr family phosphocarrier protein -
JG532_RS01465 317333..318841 - 1509 WP_001301773.1 carbohydrate kinase -
JG532_RS01470 318834..320192 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
JG532_RS01475 320269..320550 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
JG532_RS01480 320547..321020 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 306645..316132 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T184851 WP_001259385.1 NZ_CP066748:315501-315776 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T184851 NZ_CP087823:1267140-1267382 [Moraxella bovis]
ATGAATTATAGCGAATTTCGCCAAAACCTAGCCTCTGCCCTTGATTATGTTCAAGACAGCCATGCCCCTGTGATTGTCAA
ACGTGGCAAACATTCTGCGGTGGTGATTAGTCTTGATGAATATAATGCTTTTAAAGAAACAGATTATCTATTGTCAAACC
CTGCTAATGCCGAACACCTTATGCGTGGCGTGCAAGCGGTAAAAAATCGTCAACTTACCCAAAGGGATTTGATTGAGGAT
TAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT184851 WP_000593555.1 NZ_CP066748:315773-316132 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT184851 NZ_CP087823:1267372-1267629 [Moraxella bovis]
TTGAGGATTAGATGGTTTGATGAGGTATGGGAGGATTATTTGTATTGGCAAAGCCAAGATAAGAAAACCATAAAACGTAT
TAATACCTTGATTAAGGATTGCAGACGAGACCCTTTTGACGGTATCGGCAAACCCGAACCATTAAAATATAACCTAACAG
GCTACTGGTTAAGACGCATTAATGATGCCAACCGCTTGGTTTATTGTTGCGAAAGTGAAACACTCATCATTTCATGCCGT
CATCATTATGAGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References