Detailed information of TA system TA184821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 964535..965064
• Replicon: chromosome
• Accession: NZ_CP066719
• Organism: Staphylococcus xylosus strain TMW 2.1602

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JGY87_RS04480
• Protein ID: WP_029377956.1
• Coordinates: 964702..965064 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1D4YKY9
• Locus tag: JGY87_RS04475
• Protein ID: WP_017722121.1
• Coordinates: 964535..964705 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGY87_RS04450 (JGY87_04415)	960312..960791	+	480	WP_029377961.1	PH domain-containing protein	-
JGY87_RS04455 (JGY87_04420)	960784..962292	+	1509	WP_135059335.1	PH domain-containing protein	-
JGY87_RS04460 (JGY87_04425)	962279..962770	+	492	WP_029377959.1	PH domain-containing protein	-
JGY87_RS04465 (JGY87_04430)	962832..963185	+	354	WP_029377958.1	holo-ACP synthase	-
JGY87_RS04470 (JGY87_04435)	963299..964447	+	1149	WP_029377957.1	alanine racemase	-
JGY87_RS04475 (JGY87_04440)	964535..964705	+	171	WP_017722121.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JGY87_RS04480 (JGY87_04445)	964702..965064	+	363	WP_029377956.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JGY87_RS04485 (JGY87_04450)	965413..966414	+	1002	WP_029377955.1	PP2C family protein-serine/threonine phosphatase	-
JGY87_RS04490 (JGY87_04455)	966494..966820	+	327	WP_029377954.1	anti-sigma factor antagonist	-
JGY87_RS04495 (JGY87_04460)	966822..967304	+	483	WP_225489532.1	anti-sigma B factor RsbW	-
JGY87_RS04500 (JGY87_04465)	967279..968049	+	771	WP_225489533.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13539.75 Da
• Isoelectric Point: 10.3173

>T184821 WP_029377956.1 NZ_CP066719:964702-965064 [Staphylococcus xylosus]
MMRRGDVYLADLSPVQGSEQGGIRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TLDKNRLKEKLTYLSEKKMKEVNTAIDISLGLHNIRNHKS

Nucleotide

• Length: 363 bp

>T184821 NZ_CP087814:c1277597-1277415 [Moraxella bovis]
ATGAAGTATTCAGAGTTTCAAAGGTGGCTATTGGCACAAGGGGCAACGGTAAACAAGCAAGGCGGTAAAGGCTCGCACCG
TAAAGTTACCCTAAATGGCAAAACCACCACTTTCCCTTATCACGGCAGTAAAGAGATTGGGGAAGGACTAAGAAAGAAAA
TTTTAAAAGACTTGGATTTATAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6305.92 Da
• Isoelectric Point: 4.0121

>AT184821 WP_017722121.1 NZ_CP066719:964535-964705 [Staphylococcus xylosus]
MASFNQSRNHSIEQLLKEGYSQMADLNLSLAAEAFPFECEACDCNEVYISSKNNNE

Nucleotide

• Length: 171 bp

>AT184821 NZ_CP087814:c1277353-1276940 [Moraxella bovis]
ATGTACTATCCTGCTACTTTTACCCCTGATACCAATGATACTTTTTTGGTGGCGTTTCGTGATATTCCCGAAGCGGTGGG
CGTGGGTGAAACCTTTGAAATCGCCTACCAATCCGCCCTTGACGGCTTAGAAACGGCATTTAGCATCTATATGGACGAAC
GCAAGCCAATCCCCGCCCCCAGTGAGCTGTCTGACGGCGAACACGCCATTTATTTGCCTGTGGCGGTACAGACCAAACTT
GCCTTATATCACGAAATGCTAGCCCAAGGCGTTACTAAGGCGGAGCTGGCTCGCCGTCTGTCTGTCAATCAAAAACAAAT
TGATAGACTTTGGGACGTGAGCCACTCCACCAAATTAGAATTTTTGGAAAAGGCGTTTAGCGTGCTAGGCAAACGCCTAT
CCCTAGCCATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1D4YKY9