Detailed information of TA system TA184780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4664628..4665413
• Replicon: chromosome
• Accession: NZ_CP066699
• Organism: Rhodopseudomonas palustris strain RCB100

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q6N2B5
• Locus tag: I8G32_RS21305
• Protein ID: WP_011159670.1
• Coordinates: 4664628..4665125 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: I8G32_RS21310
• Protein ID: WP_042441274.1
• Coordinates: 4665126..4665413 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8G32_RS21275	4659749..4660099	+	351	WP_011159664.1	helix-turn-helix domain-containing protein	-
I8G32_RS21280	4660096..4660989	+	894	WP_011159665.1	ImmA/IrrE family metallo-endopeptidase	-
I8G32_RS21285	4661204..4661668	-	465	WP_011159666.1	ribbon-helix-helix domain-containing protein	-
I8G32_RS21290	4661678..4663672	-	1995	WP_011159667.1	conjugal transfer protein TraG	-
I8G32_RS21295	4663806..4664105	-	300	WP_011159668.1	DUF2285 domain-containing protein	-
I8G32_RS21300	4664213..4664491	+	279	WP_011159669.1	helix-turn-helix domain-containing protein	-
I8G32_RS21305	4664628..4665125	-	498	WP_011159670.1	GNAT family N-acetyltransferase	Toxin
I8G32_RS21310	4665126..4665413	-	288	WP_042441274.1	DUF1778 domain-containing protein	Antitoxin
I8G32_RS21315	4665484..4665714	-	231	Protein_4218	DUF3363 domain-containing protein	-
I8G32_RS21320	4665745..4667310	+	1566	WP_065814273.1	hypothetical protein	-
I8G32_RS21330	4667915..4668346	-	432	WP_011159672.1	phasin	-
I8G32_RS21335	4668570..4668953	-	384	WP_011159673.1	phasin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (38-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (9-84)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18315.15 Da
• Isoelectric Point: 8.8691

>T184780 WP_011159670.1 NZ_CP066699:c4665125-4664628 [Rhodopseudomonas palustris]
MTFRIEKLRREHHVEGFDCGKEPLNRFLLRYALQSQLSNSSQTYLALSQDEVVGFYTLAFGDVSYEDAPERLRKGVARHP
IPLMILARLAVASAWAGKGIGAGLLKDALARTLAAAEIAGLRAFAVHAKDDQARAFYERFDFIASPSDPMHLFVLLKDVR
ALIGP

Nucleotide

• Length: 498 bp

>T184780 NZ_CP066699:c4665125-4664628 [Rhodopseudomonas palustris]
ATGACGTTCCGCATCGAGAAGCTGCGGCGGGAGCACCACGTCGAAGGGTTTGATTGCGGCAAGGAGCCGTTAAACCGCTT
CCTGCTCCGATATGCTCTGCAAAGCCAGTTGTCCAACAGCTCGCAAACTTATCTGGCTCTCTCGCAGGACGAGGTGGTCG
GCTTCTACACCTTGGCGTTCGGGGACGTCAGTTACGAGGACGCCCCGGAGCGGCTGCGCAAGGGCGTTGCCCGGCATCCG
ATCCCGCTGATGATCCTCGCCCGCCTCGCGGTCGCCAGCGCCTGGGCCGGCAAGGGGATCGGGGCGGGCCTTCTGAAGGA
CGCGCTGGCCCGGACGCTGGCGGCGGCCGAAATCGCCGGGCTGCGGGCCTTCGCCGTCCACGCCAAGGATGATCAGGCGC
GGGCGTTCTACGAACGGTTTGATTTCATCGCGTCACCCAGCGATCCTATGCACCTCTTCGTGCTGCTGAAGGATGTCCGG
GCCTTGATCGGGCCTTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10492.91 Da
• Isoelectric Point: 5.1425

>AT184780 WP_042441274.1 NZ_CP066699:c4665413-4665126 [Rhodopseudomonas palustris]
MANAIRSEKLDLRLTPEAKRLLNAAAQASRRSVSEFVLESALDRAQEALPDRQHFGLDAAGWEAFLAALDAPARPLPRLQ
RLLTEPSVFEQAPTE

Nucleotide

• Length: 288 bp

>AT184780 NZ_CP066699:c4665413-4665126 [Rhodopseudomonas palustris]
ATGGCCAATGCCATCCGATCCGAGAAGCTCGATCTGCGGCTGACGCCGGAGGCGAAGCGCTTGCTCAACGCCGCCGCGCA
AGCGTCGCGGCGCTCGGTCAGCGAATTCGTGCTGGAGAGTGCGCTCGACCGCGCACAGGAGGCTTTGCCCGACCGTCAGC
ATTTTGGTCTCGACGCGGCGGGCTGGGAGGCGTTCCTGGCAGCACTCGATGCCCCGGCCCGGCCGCTGCCGCGGCTCCAG
CGGCTGTTGACCGAACCCAGCGTGTTCGAGCAAGCCCCGACCGAATAG

Structures

Toxin

• AlphaFold DB: Q6N2B5