Detailed information of TA system TA184776

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 11090..11697
• Replicon: plasmid p3
• Accession: NZ_CP066676
• Organism: Enterococcus faecium strain Dallas 163_1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: JCP82_RS15080
• Protein ID: WP_002321032.1
• Coordinates: 11090..11440 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: JCP82_RS15085
• Protein ID: WP_002287514.1
• Coordinates: 11434..11697 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCP82_RS15045	6259..6666	-	408	WP_002305774.1	hypothetical protein	-
JCP82_RS15050	6685..7503	-	819	WP_002305212.1	ParA family protein	-
JCP82_RS15055	7729..8022	-	294	WP_002322251.1	hypothetical protein	-
JCP82_RS15060	8158..8337	-	180	WP_001021554.1	hypothetical protein	-
JCP82_RS15065	8334..8684	-	351	WP_000568378.1	hypothetical protein	-
JCP82_RS15070	8677..10002	-	1326	WP_000969590.1	Y-family DNA polymerase	-
JCP82_RS15075	10452..11066	+	615	WP_046650770.1	recombinase family protein	-
JCP82_RS15080	11090..11440	-	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JCP82_RS15085	11434..11697	-	264	WP_002287514.1	hypothetical protein	Antitoxin
JCP82_RS15090	11935..13080	-	1146	WP_002305757.1	IS200/IS605 family element transposase accessory protein TnpB	-
JCP82_RS15095	13092..13493	-	402	WP_002305759.1	IS200/IS605 family transposase	-
JCP82_RS15100	13660..13932	-	273	WP_002348912.1	hypothetical protein	-
JCP82_RS15105	13914..14330	-	417	WP_002305764.1	hypothetical protein	-
JCP82_RS15110	14346..14597	-	252	WP_002305766.1	hypothetical protein	-
JCP82_RS15115	14597..14803	-	207	WP_002296239.1	hypothetical protein	-
JCP82_RS15120	15248..15508	-	261	WP_002321303.1	hypothetical protein	-
JCP82_RS15125	15874..16347	-	474	WP_002321302.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..23664	23664

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T184776 WP_002321032.1 NZ_CP066676:c11440-11090 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T184776 NZ_CP087803:1836862-1837140 [Moraxella bovis]
ATGATTCAAAGTTTTAAGTGCAAAGATGCCCAAAGCCTGTTTGAAACAGGGTCAAGCCGTGTATTTGCCAACTTTTTATC
CGTTGCCACTCGCAAACTTACCATGCTTGATAATGCCGTGGCACTTGCCGACCTACGCATTCCACCTGCCAACCGCCTTG
AAAAGCTGACAGGCGATAGAGAAGGTCAGTATAGTATTCGCATTAACGACCAATTTCGCATTTGCTTTGTGTGGGGCGAT
GATGGCGTGTATGATGTTGAGATTGTTGATTATCACTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT184776 WP_002287514.1 NZ_CP066676:c11697-11434 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT184776 NZ_CP087803:1837155-1837460 [Moraxella bovis]
ATGTTCAAAAATGGCATGAGACCTGTTCACGCAGGTGAGGTATTGCGTGAAGAATATCTAATCCCAATGGGTATGTCCGC
CACCGCCTTAGCAAATGCATTGGGCGTAACCCCTGCACGCATTAACGACATCGTGCGGCAGCGGCGTGGCGTTACGGCTG
ATACCGCTTTGCGTTTGGCTCGTTATTTTGGCGGTACGGCACAATTTTGGCTAAACTTACAAACCACCTATGAGCTTCGC
CTTGCCGAAACTACGCTTGGCGACAGTTTGGATTTTATCAGCCCTCATGAAGTTGCCCATGCCTAA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0