Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 518538..519109 Replicon chromosome
Accession NZ_CP066673
Organism Enterococcus faecium strain Dallas 163_1

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag JCP82_RS02475 Protein ID WP_002286801.1
Coordinates 518768..519109 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag JCP82_RS02470 Protein ID WP_002323011.1
Coordinates 518538..518768 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCP82_RS02445 514019..515347 + 1329 WP_002327434.1 FAD-containing oxidoreductase -
JCP82_RS02450 515369..515995 + 627 WP_002352507.1 cysteine hydrolase -
JCP82_RS02455 516178..516759 + 582 WP_002327433.1 TetR/AcrR family transcriptional regulator -
JCP82_RS02460 517124..517699 + 576 WP_002352505.1 SOS response-associated peptidase family protein -
JCP82_RS02465 517904..518242 - 339 WP_002306002.1 hypothetical protein -
JCP82_RS02470 518538..518768 + 231 WP_002323011.1 hypothetical protein Antitoxin
JCP82_RS02475 518768..519109 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JCP82_RS02480 519444..519901 + 458 Protein_492 transposase -
JCP82_RS02485 519996..520955 + 960 WP_002301399.1 IS30 family transposase -
JCP82_RS02490 520961..521671 + 711 Protein_494 IS3-like element IS1485 family transposase -
JCP82_RS02495 521919..522044 + 126 WP_025478972.1 LPXTG cell wall anchor domain-containing protein -
JCP82_RS02500 522118..523014 + 897 WP_002352497.1 class C sortase -
JCP82_RS02505 523195..523869 + 675 WP_002291233.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 511570..543885 32315


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T184774 WP_002286801.1 NZ_CP066673:518768-519109 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T184774 NZ_CP087803:c1618690-1618448 [Moraxella bovis]
ATGAATTATAGCGAATTTCGCCAAAACCTAGCGTCCGCCCTTGATTATGTTCAAGACAGCCATGCCCCTGTGATTGTCAA
ACGTGGCAAACATTCTGCGGTGGTGATTAGTCTTGATGAATATAATGCTTTTAAAGAAACAGATTATCTATTGTCAAACC
CTGCTAATGCCGAACACCTTATGCGTGGCGTGCAAGCGGTAAAAAATCGTCAACTTACCCAAAGGGATTTGATTGAGGAT
TAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT184774 WP_002323011.1 NZ_CP066673:518538-518768 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT184774 NZ_CP087803:c1618458-1618201 [Moraxella bovis]
TTGAGGATTAGATGGTTTGATGAGGTATGGGAGGATTATTTGTATTGGCAAAGCCAAGATAAGAAAACCATAAAACGTAT
TAATACCTTGATTAAGGATTGCAGACGAGACCCTTTTGACGGTATCGGCAAACCCGAACCATTAAAATATAACCTAACAG
GCTACTGGTTAAGACGCATTGATGATGCCAACCGCTTGGTTTATTGTTGCGAAAGTGAAACACTCATCATTTCATGCCGT
CATCATTATGAGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References