Detailed information of TA system TA184772

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 12005..12612
• Replicon: plasmid p3
• Accession: NZ_CP066670
• Organism: Enterococcus faecium strain Dallas 158

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: JCP74_RS14615
• Protein ID: WP_002321032.1
• Coordinates: 12005..12355 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: JCP74_RS14620
• Protein ID: WP_002287514.1
• Coordinates: 12349..12612 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCP74_RS14580	7298..7705	-	408	WP_002305774.1	hypothetical protein	-
JCP74_RS14585	7724..8542	-	819	WP_002305212.1	ParA family protein	-
JCP74_RS14590	8768..9061	-	294	WP_002322251.1	hypothetical protein	-
JCP74_RS14595	9073..9252	-	180	WP_002322386.1	hypothetical protein	-
JCP74_RS14600	9249..9599	-	351	WP_002303114.1	hypothetical protein	-
JCP74_RS14605	9592..10917	-	1326	WP_002348911.1	Y-family DNA polymerase	-
JCP74_RS14610	11367..11981	+	615	WP_002318230.1	recombinase family protein	-
JCP74_RS14615	12005..12355	-	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JCP74_RS14620	12349..12612	-	264	WP_002287514.1	hypothetical protein	Antitoxin
JCP74_RS14625	12850..13995	-	1146	WP_002305757.1	IS200/IS605 family element transposase accessory protein TnpB	-
JCP74_RS14630	14007..14408	-	402	WP_002305759.1	IS200/IS605 family transposase	-
JCP74_RS14635	14575..14847	-	273	WP_002348912.1	hypothetical protein	-
JCP74_RS14640	14829..15245	-	417	WP_002305764.1	hypothetical protein	-
JCP74_RS14645	15261..15512	-	252	WP_002305766.1	hypothetical protein	-
JCP74_RS14650	15512..15718	-	207	WP_002296239.1	hypothetical protein	-
JCP74_RS14655	16163..16423	-	261	WP_002321303.1	hypothetical protein	-
JCP74_RS14660	16789..17262	-	474	WP_002321302.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..23540	23540

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T184772 WP_002321032.1 NZ_CP066670:c12355-12005 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T184772 NZ_CP087803:1474751-1474933 [Moraxella bovis]
ATGAAGTATTCAGAGTTTCAAAGGTGGCTATTGGCACAAGGGGCAACGGTAAACAAGCAAGGCGGTAAAGGCTCGCACCG
TAAAGTTACCCTAAATGGCAAAACCACCACTTTCCCTTATCACGGCAGTAAAGAGATTGGGGAAGGACTAAGAAAGAAAA
TTTTAAAAGACTTGGATTTATAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT184772 WP_002287514.1 NZ_CP066670:c12612-12349 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT184772 NZ_CP087803:1474994-1475407 [Moraxella bovis]
ATGTACTATCCTGCTACTTTTACCCCTGATACCAATGATACTTTTTTGGTGGCGTTTCGTGATATTCCCGAAGCGGTGGG
CGTGGGTGAAACCTTTGAAATCGCCTACCAATCCGCCCTTGACGGCTTAGAAACGGCATTTAGCATCTATATGGACGAAC
GCAAGCCAATCCCCGCCCCCAGTGAGCTGTCTGACGGCGAACACGCCATTTATTTGCCTGTGGCGGTACAGACCAAACTT
GCCTTATATCACGAAATGCTAGCCCAAGGCGTTACTAAGGCGGAGCTGGCTCGCCGTCTGTCTGTCAATCAAAAACAAAT
TGATAGACTTTGGGACGTGAGCCACTCCACCAAATTAGAATTTTTGGAAAAGGCGTTTAGCGTGCTAGGCAAACGCCTAT
CCCTAGCCATTTAA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0