Detailed information of TA system TA184768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 15156..15763
• Replicon: plasmid p2
• Accession: NZ_CP066661
• Organism: Enterococcus faecium strain Dallas 155

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: JCP86_RS15115
• Protein ID: WP_002321032.1
• Coordinates: 15413..15763 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: JCP86_RS15110
• Protein ID: WP_002287514.1
• Coordinates: 15156..15419 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCP86_RS15070	10506..10979	+	474	WP_002321302.1	single-stranded DNA-binding protein	-
JCP86_RS15075	11345..11605	+	261	WP_002321303.1	hypothetical protein	-
JCP86_RS15080	12050..12256	+	207	WP_002296239.1	hypothetical protein	-
JCP86_RS15085	12256..12507	+	252	WP_002305766.1	hypothetical protein	-
JCP86_RS15090	12523..12939	+	417	WP_002305764.1	hypothetical protein	-
JCP86_RS15095	12921..13193	+	273	WP_002348912.1	hypothetical protein	-
JCP86_RS15100	13360..13761	+	402	WP_002305759.1	IS200/IS605 family transposase	-
JCP86_RS15105	13773..14918	+	1146	WP_002305757.1	IS200/IS605 family element transposase accessory protein TnpB	-
JCP86_RS15110	15156..15419	+	264	WP_002287514.1	hypothetical protein	Antitoxin
JCP86_RS15115	15413..15763	+	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JCP86_RS15120	15787..16401	-	615	WP_002318230.1	recombinase family protein	-
JCP86_RS15125	16851..18176	+	1326	WP_196838111.1	Y-family DNA polymerase	-
JCP86_RS15130	18169..18519	+	351	WP_002303114.1	hypothetical protein	-
JCP86_RS15135	18516..18695	+	180	WP_002322386.1	hypothetical protein	-
JCP86_RS15140	18707..19000	+	294	WP_002322251.1	hypothetical protein	-
JCP86_RS15145	19226..20044	+	819	WP_002305212.1	ParA family protein	-
JCP86_RS15150	20063..20470	+	408	WP_002305774.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..62212	62212

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T184768 WP_002321032.1 NZ_CP066661:15413-15763 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T184768 NZ_CP087803:c136803-136468 [Moraxella bovis]
ATGTTGCATTTTGCTGAAACATCATTATTCACTAAACAAATCATAGACTTAATGGATGATGACGAGTACAGACTATTACA
AACAAACTTGATGAAAAACCCACATCAAGGCGACATTGTTCGTGGTACTGGCGGAGTACGCAAAACAAGATGGTCAATTG
ATGGCAAGGGCAAAAGTGGTGGCAGTCGCATCATTTACTTTTTTGTTGATGGTGCTGGCATTTTCTTTATGCTCTTGGCT
TATCCTAAATCAAAACAAACAACGCTATCGGCGGATGAGAAAAAAGAAATGCTCAAACTAACCACCGCAATTAAGGAGAT
TTACCGTGCAAAATGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT184768 WP_002287514.1 NZ_CP066661:15156-15419 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT184768 NZ_CP087803:c136478-136197 [Moraxella bovis]
GTGCAAAATGAGTTTTACGATGATTTAAAAGCCTCATTGACTGAGGCTCTGGCAATCGCTAAGGGCGAAATTTCCCCAAG
TCGTACATTCACTTATGAACGCCCAAACATCAAAGATATTCGGGCAAAAACAGGGCTATCACAAACCCAGTTCGCCCAGA
AGCTACACATAAGCCCTAAAACGCTTAAAAACTGGGAACAGGGCATTAGAACGCCAACAGGTCCAGCCATTACCCTAATT
AGATTACTTGACAAAAACCCTGATTTAATTAGCATGGCATAA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0