Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 538230..538801 Replicon chromosome
Accession NZ_CP066659
Organism Enterococcus faecium strain Dallas 155

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag JCP86_RS02535 Protein ID WP_002286801.1
Coordinates 538460..538801 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag JCP86_RS02530 Protein ID WP_002323011.1
Coordinates 538230..538460 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCP86_RS02500 533363..534694 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
JCP86_RS02505 534716..535342 + 627 WP_002286816.1 cysteine hydrolase -
JCP86_RS02510 535525..536106 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
JCP86_RS02515 536838..537185 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
JCP86_RS02520 537161..537400 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
JCP86_RS02525 537605..537943 - 339 WP_002286804.1 hypothetical protein -
JCP86_RS02530 538230..538460 + 231 WP_002323011.1 hypothetical protein Antitoxin
JCP86_RS02535 538460..538801 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JCP86_RS02540 539834..541021 - 1188 WP_002296840.1 IS256-like element IS16 family transposase -
JCP86_RS02545 541815..542009 + 195 WP_002297028.1 hypothetical protein -
JCP86_RS02550 542167..542449 + 283 Protein_506 transposase -
JCP86_RS02555 542693..543523 - 831 WP_002286789.1 manganese catalase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 539834..541021 1187


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T184766 WP_002286801.1 NZ_CP066659:538460-538801 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T184766 NZ_CP087800:c2226692-2226504 [Moraxella bovis]
ATGCCCCCAAGTAGCGATGAGATGATAAAGCGAATTACCGAAGACGGTTGGTATTTTGTAAAACAAAAAGGCTCTCACAA
GCACTACAAACACCCAACCAAAGAAGGTAAAGTAACAATCCCAAACGGTCGTGAAAACCTACCCAAAGGAACGCTAAAAA
GCATCATCAAACAAGCAGGGTTGGATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT184766 WP_002323011.1 NZ_CP066659:538230-538460 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT184766 NZ_CP087800:c2226441-2226034 [Moraxella bovis]
ATGTTATTTCCCATTGCCATTGAAAAACCTGCCAATGAAAACGAATGTTATGGCGTGATTGTGCCAGACATTGCAGGGTG
TTTTAGTGCAGGCGATACACCTGATGAAGCCATTAAGAATGCTTATGAAGCCATCAGTTTGCATTTGGAAGGCATGGCAG
AAGATGGCGATACACTACCTACCGCCAAGCCCATTTTTTATCATCAAGACAATAGCGAATTTGACGGTATGTTGTGGGCA
GTGGTGGACGTGGATATGTCTGCCTTTATGGGTAAGGCGGAGAAAATCAACGTTACCTTGCCAAGCCTACTCATCACCAA
AATTGACGAAAAAGTCTTGTCTCATAAATCGCTATACAAAAGCCGTTCTAATTATTTGGCACAATTGGCGATGGCAGATT
TGGGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References