Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 516440..517011 Replicon chromosome
Accession NZ_CP066653
Organism Enterococcus faecium strain Dallas 154_1

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag JCP65_RS02460 Protein ID WP_002286801.1
Coordinates 516670..517011 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag JCP65_RS02455 Protein ID WP_002323011.1
Coordinates 516440..516670 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCP65_RS02430 511921..513249 + 1329 WP_002327434.1 FAD-containing oxidoreductase -
JCP65_RS02435 513271..513897 + 627 WP_002352507.1 cysteine hydrolase -
JCP65_RS02440 514080..514661 + 582 WP_002327433.1 TetR/AcrR family transcriptional regulator -
JCP65_RS02445 515026..515601 + 576 WP_002352505.1 SOS response-associated peptidase family protein -
JCP65_RS02450 515806..516144 - 339 WP_002306002.1 hypothetical protein -
JCP65_RS02455 516440..516670 + 231 WP_002323011.1 hypothetical protein Antitoxin
JCP65_RS02460 516670..517011 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JCP65_RS02465 517346..517803 + 458 Protein_489 transposase -
JCP65_RS02470 517898..518857 + 960 WP_002301399.1 IS30 family transposase -
JCP65_RS02475 518863..519573 + 711 Protein_491 IS3-like element IS1485 family transposase -
JCP65_RS02480 519821..519946 + 126 WP_025478972.1 LPXTG cell wall anchor domain-containing protein -
JCP65_RS02485 520020..520916 + 897 WP_002352497.1 class C sortase -
JCP65_RS02490 521097..521771 + 675 WP_002291233.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 484479..596082 111603


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T184764 WP_002286801.1 NZ_CP066653:516670-517011 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T184764 NZ_CP087800:c1964734-1964399 [Moraxella bovis]
ATGTGCGAATTAATGTATGAATTAGAATTTGACGAGCGAGCCTTAAAAGAATGGCAAAAACTGGGCGACACCATAAAAAC
GCAGTTTAAAAAGAGACTGGCTAAGGTGCTAAAAAATCCGCACATCATCGCCAATCGCCTAAGCGGTGGACAAAATTTAT
ACAAAATCAACTTCCGCTCGGCAGGTTATCGCCTTGTGTATCAAGTTGAAGATGATAAAGTGGTGGTGTTTGTCATCGTG
ATGGGTAAGCGTGAGAAAAATGCCGTCTATGACACCGCCGAAGAATGGCTTGCTGAACGTCAATCGGACAATCAAGACAC
ACAATCTGTCAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT184764 WP_002323011.1 NZ_CP066653:516440-516670 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT184764 NZ_CP087800:c1964976-1964734 [Moraxella bovis]
ATGGCAAATATCGTGGCTCGTGTGGACGATGACCTAAAAGCCCAATCATTTTTGGTACTGCAAGAACTTGGCGTGACACC
GTCTGAGCTTATCCGCCAAACCTTGCAATATGTGGCTTATCACAAAAAATTACCCTTTAAGACGGTGGTTTTGACCGATG
AAGACAGTGAGCTTTTAGCCCTTGCCAAAGAACGCCTTGATAATCCTGCCCCACGCATCAAAGTCAGTTTGGACGAGCTT
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References