Detailed information of TA system TA184749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 42550..43157
• Replicon: plasmid p2
• Accession: NZ_CP066631
• Organism: Enterococcus faecium strain Dallas 137

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: JCP61_RS14890
• Protein ID: WP_002321032.1
• Coordinates: 42550..42900 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: JCP61_RS14895
• Protein ID: WP_002287514.1
• Coordinates: 42894..43157 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCP61_RS14855	37843..38250	-	408	WP_002305774.1	hypothetical protein	-
JCP61_RS14860	38269..39087	-	819	WP_002305212.1	ParA family protein	-
JCP61_RS14865	39313..39606	-	294	WP_002322251.1	hypothetical protein	-
JCP61_RS14870	39618..39797	-	180	WP_002322386.1	hypothetical protein	-
JCP61_RS14875	39794..40144	-	351	WP_002303114.1	hypothetical protein	-
JCP61_RS14880	40137..41462	-	1326	WP_002348911.1	Y-family DNA polymerase	-
JCP61_RS14885	41912..42526	+	615	WP_002318230.1	recombinase family protein	-
JCP61_RS14890	42550..42900	-	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JCP61_RS14895	42894..43157	-	264	WP_002287514.1	hypothetical protein	Antitoxin
JCP61_RS14900	43395..44540	-	1146	WP_002305757.1	IS200/IS605 family element transposase accessory protein TnpB	-
JCP61_RS14905	44552..44953	-	402	WP_002305759.1	IS200/IS605 family transposase	-
JCP61_RS14910	45120..45392	-	273	WP_002348912.1	hypothetical protein	-
JCP61_RS14915	45374..45790	-	417	WP_002305764.1	hypothetical protein	-
JCP61_RS14920	45806..46057	-	252	WP_002305766.1	hypothetical protein	-
JCP61_RS14925	46057..46263	-	207	WP_002296239.1	hypothetical protein	-
JCP61_RS14930	46708..46968	-	261	WP_002321303.1	hypothetical protein	-
JCP61_RS14935	47334..47807	-	474	WP_002321302.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..61213	61213

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T184749 WP_002321032.1 NZ_CP066631:c42900-42550 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T184749 NZ_CP087798:c1623730-1623488 [Moraxella bovis]
ATGAATTATAGCGAATTTCGCCAAAACCTAGCGTCCGCCCTTGATTATGTTCAAGACAGCCATGCCCCTGTGATTGTCAA
ACGTGGCAAACATTCTGCGGTGGTGATTAGTCTTGATGAATATAATGCTTTTAAAGAAACAGATTATCTATTGTCAAACC
CTGCTAATGCCGAACACCTTATGCGTGGCGTGCAAGCGGTAAAAAATCGTCAACTTACCCAAAGGGATTTGATTGAGGAT
TAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT184749 WP_002287514.1 NZ_CP066631:c43157-42894 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT184749 NZ_CP087798:c1623498-1623241 [Moraxella bovis]
TTGAGGATTAGATGGTTTGATGAGGTATGGGAGGATTATTTGTATTGGCAAAGCCAAGATAAGAAAACCATAAAACGTAT
TAATACCTTGATTAAGGATTGCAGACGAGACCCTTTTGACGGTATCGGCAAACCCGAACCATTAAAATATAACCTAACAG
GCTACTGGTTAAGACGCATTGATGATGCCAACCGCTTGGTTTATTGTTGCGAAAGTGAAACACTCATCATTTCATGCCGT
CATCATTATGAGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0