Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 520956..521527 Replicon chromosome
Accession NZ_CP066629
Organism Enterococcus faecium strain Dallas 137

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag JCP61_RS02480 Protein ID WP_002286801.1
Coordinates 521186..521527 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag JCP61_RS02475 Protein ID WP_002323011.1
Coordinates 520956..521186 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCP61_RS02450 516437..517765 + 1329 WP_002327434.1 FAD-containing oxidoreductase -
JCP61_RS02455 517787..518413 + 627 WP_002352507.1 cysteine hydrolase -
JCP61_RS02460 518596..519177 + 582 WP_002327433.1 TetR/AcrR family transcriptional regulator -
JCP61_RS02465 519542..520117 + 576 WP_002352505.1 SOS response-associated peptidase family protein -
JCP61_RS02470 520322..520660 - 339 WP_002306002.1 hypothetical protein -
JCP61_RS02475 520956..521186 + 231 WP_002323011.1 hypothetical protein Antitoxin
JCP61_RS02480 521186..521527 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JCP61_RS02485 521862..522319 + 458 Protein_493 transposase -
JCP61_RS02490 522414..523373 + 960 WP_002301399.1 IS30 family transposase -
JCP61_RS02495 523379..524089 + 711 Protein_495 IS3-like element IS1485 family transposase -
JCP61_RS02500 524337..524462 + 126 WP_025478972.1 LPXTG cell wall anchor domain-containing protein -
JCP61_RS02505 524536..525432 + 897 WP_002352497.1 class C sortase -
JCP61_RS02510 525613..526287 + 675 WP_002291233.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 518596..525432 6836


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T184748 WP_002286801.1 NZ_CP066629:521186-521527 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T184748 NZ_CP087798:c1623498-1623241 [Moraxella bovis]
TTGAGGATTAGATGGTTTGATGAGGTATGGGAGGATTATTTGTATTGGCAAAGCCAAGATAAGAAAACCATAAAACGTAT
TAATACCTTGATTAAGGATTGCAGACGAGACCCTTTTGACGGTATCGGCAAACCCGAACCATTAAAATATAACCTAACAG
GCTACTGGTTAAGACGCATTGATGATGCCAACCGCTTGGTTTATTGTTGCGAAAGTGAAACACTCATCATTTCATGCCGT
CATCATTATGAGGCTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT184748 WP_002323011.1 NZ_CP066629:520956-521186 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT184748 NZ_CP087798:c1623730-1623488 [Moraxella bovis]
ATGAATTATAGCGAATTTCGCCAAAACCTAGCGTCCGCCCTTGATTATGTTCAAGACAGCCATGCCCCTGTGATTGTCAA
ACGTGGCAAACATTCTGCGGTGGTGATTAGTCTTGATGAATATAATGCTTTTAAAGAAACAGATTATCTATTGTCAAACC
CTGCTAATGCCGAACACCTTATGCGTGGCGTGCAAGCGGTAAAAAATCGTCAACTTACCCAAAGGGATTTGATTGAGGAT
TAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References