Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2166838..2167409 Replicon chromosome
Accession NZ_CP066614
Organism Enterococcus faecium strain Dallas 124_3

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag JCP92_RS10530 Protein ID WP_002286801.1
Coordinates 2166838..2167179 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag JCP92_RS10535 Protein ID WP_002323011.1
Coordinates 2167179..2167409 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCP92_RS10505 2162048..2163382 - 1335 WP_002286788.1 ABC transporter substrate-binding protein -
JCP92_RS10510 2163590..2164420 + 831 WP_002286789.1 manganese catalase family protein -
JCP92_RS10515 2164664..2164946 - 283 Protein_2054 transposase -
JCP92_RS10520 2165104..2165298 - 195 WP_002297028.1 hypothetical protein -
JCP92_RS10525 2165803..2165988 - 186 WP_002304207.1 hypothetical protein -
JCP92_RS10530 2166838..2167179 - 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JCP92_RS10535 2167179..2167409 - 231 WP_002323011.1 hypothetical protein Antitoxin
JCP92_RS10540 2167696..2168034 + 339 WP_002286804.1 hypothetical protein -
JCP92_RS10545 2168239..2168478 - 240 WP_002321938.1 SOS response-associated peptidase family protein -
JCP92_RS10550 2168454..2168801 - 348 WP_002286808.1 SOS response-associated peptidase family protein -
JCP92_RS10555 2169533..2170114 - 582 WP_010729547.1 TetR/AcrR family transcriptional regulator -
JCP92_RS10560 2170297..2170923 - 627 WP_002286816.1 cysteine hydrolase -
JCP92_RS10565 2170945..2172276 - 1332 WP_002286818.1 FAD-containing oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T184735 WP_002286801.1 NZ_CP066614:c2167179-2166838 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T184735 NZ_CP087796:362337-362729 [Moraxella bovis]
ATGAAATATTTGCTGGACACCAATGCAGTCATTGCGATTTTGAATGCCAGTGAACGATTTTTGACCACGCTAGAACGGCA
TAAAGAGCGTGAAATTGCCATCTCGTCAATTGTTTTGTCGGAGCTGTATTATGGGGCGTACAAAAGCCAAAAAACCGCCC
AAAACCTTGAAAAAATCAATCTACTACCCTTTGAAGTGTTGCAATTTAATTCACAGGACGCTGATAAGGCTGGCGAAATT
GGTGCAACCCTAGAAAGGCGTGGCACACCCATTGGTTCTTATGACACGCTGATTGCAGGGCAAGCGATTGCCAATGATTT
GATTGTTATTACTGACAATGTGCGAGAATTTCTCCGAGTGGACGGTTTGCAAATTGAAAATTGGTTAAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT184735 WP_002323011.1 NZ_CP066614:c2167409-2167179 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT184735 NZ_CP087796:362077-362340 [Moraxella bovis]
ATGCGCCAAACTGCCACCGCAAAATTATTTTGGACTGGCAACAGTCAAGCCGTTCGCTTACCCAAAGCCTTTCGTTTTGA
AGGCACGGAAGTGCGTATCAAAAAACAAGGACGACAAGTGATTATTGAGCCAGCAGAGACCCAAGATTGGGCGTGGCTGG
ACAAATTGCGAGCAATGCCAAAAGATGACAGTTTTGAAGAAGCCATTTTGGAAGGTCGCCAAAATTTAAAAGCCGATGAA
CGAGATTGGGGTGCATTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References