Detailed information of TA system TA184734

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 30581..31100
• Replicon: plasmid p124_1_37kb
• Accession: NZ_CP066610
• Organism: Enterococcus faecium strain Dallas 124_1

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: JCP68_RS15295
• Protein ID: WP_000588503.1
• Coordinates: 30843..31100 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: JCP68_RS15290
• Protein ID: WP_000388479.1
• Coordinates: 30581..30850 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCP68_RS15255	26151..26567	-	417	WP_001232859.1	hypothetical protein	-
JCP68_RS15260	26570..26854	-	285	WP_000353844.1	hypothetical protein	-
JCP68_RS15265	27200..27445	+	246	WP_002311817.1	hypothetical protein	-
JCP68_RS15270	27747..27842	-	96	WP_106913803.1	type I toxin-antitoxin system Fst family toxin	-
JCP68_RS15275	28568..29248	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
JCP68_RS15280	29324..29890	+	567	Protein_37	hypothetical protein	-
JCP68_RS15285	30271..30450	+	180	WP_001120991.1	hypothetical protein	-
JCP68_RS15290	30581..30850	+	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JCP68_RS15295	30843..31100	+	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
JCP68_RS15300	31737..32561	+	825	WP_001809248.1	hypothetical protein	-
JCP68_RS15305	32726..33340	-	615	WP_001261742.1	recombinase family protein	-
JCP68_RS15310	33790..35115	+	1326	WP_000969590.1	Y-family DNA polymerase	-
JCP68_RS15315	35108..35458	+	351	WP_000568378.1	hypothetical protein	-
JCP68_RS15320	35455..35634	+	180	WP_001021554.1	hypothetical protein	-
JCP68_RS15325	35770..36060	+	291	WP_000026576.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B) / aph(3')-III / VanHAX	-	1..37877	37877

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T184734 WP_000588503.1 NZ_CP066610:30843-31100 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T184734 NZ_CP087796:359243-359515 [Moraxella bovis]
GTGGCTAAACGTAGTTTTGAAATGGGCAATCCCTTTAAAAGGGATTTAAAAAAGCATTATTTGGCATTGATTGGCGAAGC
GTGGACGGAAGTGTCTGATTGCTTGTTAAATGCCAAACCCATACCTCAAAAATACCAAGACCACCCATTGCAAGGCGATT
GGGTGGGGTTTAGAGATTGTCATATCAAGCCTGATTTGGTGCTAATTTATAAAATAAATGATGAAGCGGTGGAATTACAT
CGGCTTGCCACGCATAGCGAGTTGTTTGGTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT184734 WP_000388479.1 NZ_CP066610:30581-30850 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT184734 NZ_CP087796:358957-359250 [Moraxella bovis]
ATGAGCCACACCAATTTTAATATGCGACTAGACAACGACCTACGCCAAAAAGTCTATCCTGTGCTAGAACAATACGGCTT
GACCCCAAGCCAAGCAGTGCGGATGTTTTTTAATCAAATCGCCCAAACAGGCAAAGTGCCATTGTCTTTTGATTGGGGGC
AGTCGCATACGCTTAGTAGCAATGGCGAAAAGCTGTTGCGTGAGAGTATTCAAGCTTTTGAAAATGGCGAAACCGAGCGA
TTTGATAGCTTAGATGAGTTAAATCAAGCAATGGCGGAGATTGCTCGTGGCTAA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7