Detailed information of TA system TA184727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 50460..51067
• Replicon: plasmid p111_212kb
• Accession: NZ_CP066600
• Organism: Enterococcus faecium strain Dallas 111

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: JCP62_RS14030
• Protein ID: WP_002321032.1
• Coordinates: 50717..51067 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: JCP62_RS14025
• Protein ID: WP_002287514.1
• Coordinates: 50460..50723 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCP62_RS13985	45483..45743	+	261	WP_002321303.1	hypothetical protein	-
JCP62_RS13990	46188..46394	+	207	WP_002296239.1	hypothetical protein	-
JCP62_RS13995	46394..46645	+	252	WP_002305766.1	hypothetical protein	-
JCP62_RS14000	46661..47077	+	417	WP_002305764.1	hypothetical protein	-
JCP62_RS14005	47059..47694	+	636	WP_002305763.1	hypothetical protein	-
JCP62_RS14010	48075..48254	+	180	WP_002305761.1	hypothetical protein	-
JCP62_RS14015	48664..49065	+	402	WP_002305759.1	IS200/IS605 family transposase	-
JCP62_RS14020	49077..50222	+	1146	WP_002305757.1	IS200/IS605 family element transposase accessory protein TnpB	-
JCP62_RS14025	50460..50723	+	264	WP_002287514.1	hypothetical protein	Antitoxin
JCP62_RS14030	50717..51067	+	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JCP62_RS14035	51091..51705	-	615	WP_002318230.1	recombinase family protein	-
JCP62_RS14040	52019..52441	+	423	WP_002288787.1	hypothetical protein	-
JCP62_RS14045	52451..52654	+	204	WP_002299573.1	hypothetical protein	-
JCP62_RS14050	52865..53449	+	585	WP_002299575.1	recombinase family protein	-
JCP62_RS14055	53621..54301	-	681	WP_199023549.1	IS6 family transposase	-
JCP62_RS14060	54376..54744	+	369	Protein_56	aminoglycoside phosphotransferase family protein	-
JCP62_RS14065	54782..55954	-	1173	WP_002349987.1	IS256-like element IS1542 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..212766	212766
	-	inside	IScluster/Tn	-	-	48664..57256	8592

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T184727 WP_002321032.1 NZ_CP066600:50717-51067 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T184727 NZ_CP087793:1163506-1163748 [Moraxella bovis]
ATGAATTATAGCGAATTTCGCCAAAACCTAGCGTCCGCCCTTGATTATGTTCAAGACAGCCATGCCCCTGTGATTGTCAA
ACGTGGCAAACATTCTGCGGTGGTGATTAGTCTTGATGAATATAATGCTTTTAAAGAAACAGATTATCTATTGTCAAACC
CTGCTAATGCCGAACACCTTATGCGTGGCGTGCAAGCGGTAAAAAATCGTCAACTTACCCAAAGGGATTTGATTGAGGAT
TAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT184727 WP_002287514.1 NZ_CP066600:50460-50723 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT184727 NZ_CP087793:1163738-1163995 [Moraxella bovis]
TTGAGGATTAGATGGTTTGATGAGGTATGGGAGGATTATTTGTATTGGCAAAGCCAAGATAAGAAAACCATAAAACGTAT
TAATACCTTGATTAAGGATTGCAGACGAGACCCTTTTGACGGTATCGGCAAACCCGAACCATTAAAATATAACCTAACAG
GCTACTGGTTAAGACGCATTGATGATGCCAACCGCTTGGTTTATTGTTGCGAAAGTGAAACACTCATCATTTCATGCCGT
CATCATTATGAGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0