Detailed information of TA system TA184711

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 25314..25921
• Replicon: plasmid p100_1_56kb
• Accession: NZ_CP066578
• Organism: Enterococcus faecium strain Dallas 100_1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: JCP75_RS15490
• Protein ID: WP_002321032.1
• Coordinates: 25571..25921 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: JCP75_RS15485
• Protein ID: WP_002287514.1
• Coordinates: 25314..25577 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCP75_RS15445	20664..21137	+	474	WP_002321302.1	single-stranded DNA-binding protein	-
JCP75_RS15450	21503..21763	+	261	WP_002321303.1	hypothetical protein	-
JCP75_RS15455	22208..22414	+	207	WP_002296239.1	hypothetical protein	-
JCP75_RS15460	22414..22665	+	252	WP_002305766.1	hypothetical protein	-
JCP75_RS15465	22681..23097	+	417	WP_002305764.1	hypothetical protein	-
JCP75_RS15470	23079..23351	+	273	WP_002348912.1	hypothetical protein	-
JCP75_RS15475	23518..23919	+	402	WP_002305759.1	IS200/IS605 family transposase	-
JCP75_RS15480	23931..25076	+	1146	WP_002305757.1	IS200/IS605 family element transposase accessory protein TnpB	-
JCP75_RS15485	25314..25577	+	264	WP_002287514.1	hypothetical protein	Antitoxin
JCP75_RS15490	25571..25921	+	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JCP75_RS15495	25945..26559	-	615	WP_002318230.1	recombinase family protein	-
JCP75_RS15500	26873..27295	+	423	WP_002288787.1	hypothetical protein	-
JCP75_RS15505	27305..27508	+	204	WP_002299573.1	hypothetical protein	-
JCP75_RS15510	27719..28303	+	585	WP_002299575.1	recombinase family protein	-
JCP75_RS15515	28472..29797	+	1326	WP_085803944.1	Y-family DNA polymerase	-
JCP75_RS15520	29790..30140	+	351	WP_002303114.1	hypothetical protein	-
JCP75_RS15525	30137..30316	+	180	WP_002322386.1	hypothetical protein	-
JCP75_RS15530	30328..30621	+	294	WP_002322251.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..56293	56293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T184711 WP_002321032.1 NZ_CP066578:25571-25921 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T184711 NZ_CP087791:200309-200611 [Moraxella bovis]
ATGACCAAGTCTGATAAATTAAAAGCCAAATTGTATGGCGATGTACGAACTTTTGTATTTAGTGATTTGGTTACTTTATT
ATCGCAATTAGGTTATGAAATGCACGAACGGGCAGGTTCTCGTGTGGTATTTGTTCATTGTGATGATAATAGCGACCGCA
TTCATTTGCATAAACCCCACCCCGAAAATACCATTAAAGGTGGAGCATTAAAAGCGGTCAAAGCCTATTTGTCCGAAAAA
GGCTATTTTGATGAATTTAATGATGATGTGTTAAATCAGGAGCTTAAAAATGATGACCTATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT184711 WP_002287514.1 NZ_CP066578:25314-25577 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT184711 NZ_CP087791:200598-200933 [Moraxella bovis]
ATGATGACCTATAAAGGCTATGTTGGCGAATTTACGCTTGATAATGATGATGAAATATTTTATGGTAAACTTATTGGCAT
TAATCAGCTTGTTACTTTTGAAGCAGACAATGCCCATGATTTAAAACAAGCCTTTTATGACAGCGTGGACGATTATTTGG
CGTTTTGTGCTGAACAAGGCATAGAGCCTGATAAGCCTTTTAAGGGTTCATTTAATGTGCGTATTGATAGCGAATTGCAC
CGCCGTGCTTATATGGCAAGCCGTGACAGTAGTTTAAATGCGTTTGTAGGACAAGCCATTAGTGAAAAGCTAAATAGAAT
GGCTGTATCAATATAA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0